子宫颈腺癌中HPV感染与发病年龄的临床分析

Clinical analysis of HPV infection in cervical adenocarcinoma and its relationship with age of onset

目的探讨子宫颈腺癌中人乳头状瘤病毒(human papillomavirus,HPV)型别感染及其与发病年龄的关系。方法收集江西省妇幼保健院存档的子宫颈腺癌标本120例,采用三明治技术进行蜡块切片,应用SPF10-PCR技术进行DNA扩增,使用反向杂交线型探针检测技术(Li PA)的方法进行HPV分型,对所有切片进行病理阅片和诊断,分析子宫颈腺癌标本的HPV感染情况。结果 120例子宫颈腺癌标本中,HPV阳性率为77.5%(93/120)。子宫颈腺癌中HPV 16、18均为其主要型别,HPV16、18在子宫颈腺癌HPV阳性患者中均占43.01%(40/93)。感染HPV 16、18患者的平均年龄分别为43.41、41.06岁,感染HPV其他型别的患者平均年龄为46.85岁,差异有统计学意义(F=3.58,P=0.032)。结论 HPV 16、18为子宫颈腺癌的主要型别,腺癌中HPV 18感染的患者较其他型别感染的年龄小。

Purpose To discuss the relationship between age of onset and the HPV types infection in cervical adenocarcinoma. Meth-ods This study included 120 specimens of cervical adenocarcinoma in Jiangxi Maternal and Child Health Hospital. The specimen blocks were sliced by sandwich method. DNA was amplified by SPF10-PCR method. DNA genotyping was used with LiPA method. All biopsy specimens had pathologic diagnosis. The HPV infection of cervical adenocarcinoma specimens was analyzed. Results 120 ade-nocarcinoma cases were included in this study. HPV positive rate was 77. 5% （93/120）. HPV 18 was the most prevalent type, which took up 43. 01% （40/93） of the total HPV positive cases, as same as HPV 16, which took up 43. 01%（40/93） of the total HPV pos-itive cases. The average ages of HPV 16 and HPV 18 were 43. 41 and 41. 06 years old respectively, other HPV types were 46. 85 years old, which showed statistically difference （F=3. 58, P=0. 032）. Conclusion HPV 18 and HPV 16 are two most prevalent HPV types in cervical adenocarcinoma. The cases of HPV 18 infection are younger than other HPV-types infection.