摘要
目的探讨TPX2蛋白在乳腺癌中的表达,分析其表达与临床病理特征的关系及其在乳腺癌治疗和预后中的意义。方法应用免疫组化SP法检测乳腺癌、癌旁和正常乳腺组织中TPX2蛋白的表达;应用RT-PCR技术检测乳腺癌、癌旁和正常乳腺组织中TPX2 mRNA的表达。结果 TPX2蛋白在乳腺癌组织中的表达(75.86%)显著高于癌旁(40.00%)及正常乳腺组织(5.00%)(P<0.01),且TPX2蛋白表达与患者年龄、临床分期无明显相关性(P>0.05);与组织学分级、淋巴结转移呈显著相关(P<0.01)。TPX2 mRNA在乳腺癌组织中的表达(1.465 7±0.136 6)也显著高于癌旁(0.923 3±0.114 8)及正常乳腺组织(0.326 9±0.097 3)(P<0.01)。结论 TPX2蛋白在乳腺癌中高表达,与乳腺癌组织学分级及淋巴结转移有一定的相关性,可能是乳腺癌发生、发展及淋巴结转移的危险因素。
Purpose To investigate the expression of TPX2 in breast cancer, analyze the correlation between its expression and clinico-pathologic characteristics and explore the significances of TPX2 in the treatment and prognosis of breast cancer. Methods Expression of TPX2 in breast cancer tissues, adjacent tissue of cancer and normal breast tissue was examined by immunohistochenistry SP method. The mRNA expression of TPX2 in breast cancer tissues, adjacent tissue of cancer and normal breast tissue was detected by reverse-transcription polymerase chain reaction （ RT-PCR） . Results The expression of TPX2 is significantly higher in breast cancer tissues （75. 86%） compared with adjacent tissue of cancer （40. 00%） and normal breast tissue （5. 00%）. And the expression of TPX2 cor-related with tumor grade and lymph node metastasis （P〈0. 01）, while unrelated to age and clinical stage （P〉0. 05）. The expression of TPX2 mRNA is also significantly higher in breast cancer tissues （ 1. 465 7 ± 0. 136 6 ） compared with adjacent tissue of cancer （0. 923 3 ±0. 114 8） and normal breast tissue （0. 326 9 ±0. 097 3） （P〈0. 01）. Conclusion High expression of TPX2 in breast cancer correlated with tumor grade and lymph node metastasis, so TPX2 might be a risk factor in the tumorigenesis, progression and lymph node metastasis in breast cancer.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2015年第5期528-531,共4页
Chinese Journal of Clinical and Experimental Pathology
基金
重庆市卫生局医学科研计划(2012-2-375)
