LbL法制备磁性纳米复合纤维膜生物相容性的研究

Biocompatibility of magnetic nanofibrous composite scaffolds fabricated by Layer-by-Layer assembly

目的将磁性纳米材料嫁接到静电纺丝纤维膜上以达到增强其生物相容性的目的。方法通过"层-层自组装"(Lb L)的方法将Fe3O4磁性纳米颗粒组装到聚乳酸-羟基乙酸共聚物(poly(lactic-co-glycolic acid),PLGA)、ε-聚己内酯(ε-caprolactone,PCL)、明胶(gelatin,Gel)共混静电纺丝纤维膜(PPG)上,使用接触角仪、电子万能试验机、扫描电镜和振动样品磁强计对其表面形貌、亲水性、拉伸性能及磁学性能进行测试。将MC3T3-E1细胞接种到复合膜上,通过CCK-8法测试其细胞增殖,并与对照组进行比较。结果扫描电镜显示Fe3O4组装到膜表面,膜仍然保持类似细胞外基质的疏松纳米纤维结构。振动样品磁强计结果显示PPG膜在修饰了Fe3O4磁性纳米颗粒后具备超顺磁性性能。接触角及吸水率测量表明PPG-F膜亲水性显著改善,CCK-8检测结果显示PPG-F膜细胞增殖活性显著高于对照组PPG膜(P<0.05)。结论本方法形成的PPG-F纳米纤维复合膜具有良好生物相容性。

Objective To improve the biocompatibility of nanofibrous scaffolds by grafting magnetic nano-particles onto them. Meth- ods Poly （ lactie-co-glycolic acid） （PLGA）/ε-caprolactone （FCL）/gelatin （Gel） nanofibrous composite membranes （PPG） were fabricated by electrospinning. And then paramagnetic Fe304 nano-particles were assembled to the scaffolds by layer-by-layer （LbL） process. Contact angle meter, electronic universal testing machine, vibrating sample magnetometer were used to characterize the surface topography,hydrophilicity,tensile strength and magnetic properties. Osteoblastic cell line MC3T3-E1 was seeded onto the scaffolds. CCK-8 was used to detect the cell proliferation. Results FESEM showed the structure of the PPG-F scafffold was similar to that of ex- tracellular matrix,and Fe304 particles were successfully grafted onto the scaffolds. Vibrating sample magnetometer results indicated that after being coated with Fe304 particles,PPG-F membrane was of super paramagnetic property. Both improved hydrophilicity and better cell proliferation were observed in PPG-F group （P 〈 0.05）. Conclusions Grafting magnetic Fe304 nano-particles onto nanofibrous scaffolds can obviously improve the biocompatibility.