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组蛋白H2A去泛素化酶MYSM1缺陷小鼠造血干细胞增殖加快且凋亡增加 被引量：2

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摘要 目的研究组蛋白H2A去泛素化酶MYSM1缺陷(MYSM1-/-)小鼠骨髓造血干细胞(HSC)的静息状态、增殖与凋亡情况。方法分离MYSM1-/-小鼠和野生对照(MYSM1+/+)小鼠的骨髓细胞,利用免疫磁珠和流式细胞仪分选获得表面标志为lin-Sca-1+c-kit+的HSC。采用腹腔注射溴脱氧尿嘧啶核苷(Brd U)的方法检测HSC增殖与细胞周期、采用Hoechst 33342-派若宁(pyronin)Y双染色法检测静息态HSC的比例、采用异硫氰酸荧光素标记膜联素Ⅴ/7-氨基放线菌素D(annexinⅤ-FITC/7-AAD)双染色法检测HSC凋亡。比较MYSM1-/-小鼠和MYSM1+/+对照小鼠HSC数目、细胞周期、细胞增殖和细胞凋亡的变化情况。结果 MYSM1-/-小鼠的骨髓细胞、HSC总数与野生型小鼠相比明显降低;HSC的S期比例增加、增殖加快、静息期细胞减少但功能有缺陷的MYSM1-/-HSC凋亡率显著上升。结论 MYSM1对维持HSC的静息状态有非常重要的作用,其缺陷可引起静息态的HSC大量进入S期,异常增殖的HSC凋亡增加并最终引起HSC和骨髓细胞总数减少。
作者 刘莹 陈旭 杨安钢 王涛
机构地区 第四军医大学基础医学院免疫学教研室 第四军医大学基础医学院遗传与发育生物学教研室
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2015年第7期937-940,共4页 Chinese Journal of Cellular and Molecular Immunology
基金 国家自然科学基金(81172289)
关键词 组蛋白H2A去泛素化 MYSM1 造血干细胞 细胞增殖 细胞凋亡
分类号 R392.12 [医药卫生—免疫学] Q527.3 [生物学—生物化学]
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参考文献24

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二级参考文献15

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细胞与分子免疫学杂志
2015年 第7期

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