胶原诱导性关节炎模型大鼠炎性细胞因子表达及白喉乌头的影响

Expression of inflammatory cytokines and effect of Aconitum leucostomum Worosch. in collagen-induced arthritis rats

背景:目前治疗类风湿关节炎主要的药物有慢作用抗风湿药、生物制剂及植物药物,植物药因价格便宜、不良反应小而越来越受到瞩目。目的:观察胶原诱导性关节炎模型大鼠滑膜中肿瘤坏死因子α、白细胞介素1β表达及白喉乌头对其表达的影响。方法:将45只大鼠随机分为正常对照组8只、造模组37只,造模组于大鼠足跖及尾根部多点皮内注射牛Ⅱ型胶原乳剂建立胶原诱导性关节炎大鼠模型。造模成功后,随机选取24只造模成功大鼠进行后续实验,将其随机分为模型组、雷公藤组和白喉乌头组,每组各8只,每周对3个造模后干预组和正常对照组共4组大鼠进行关节炎评分。经过灌胃给药治疗4周后,通过免疫组织化学染色检测肿瘤坏死因子α、白细胞介素1β在滑膜中的表达。结果与结论:与模型组比较,治疗后白喉乌头组、雷公藤组关节炎评分明显降低(P<0.05)。肿瘤坏死因子α、白细胞介素1β在模型组滑膜中表达明显高于正常对照组(P<0.05),在白喉乌头组、雷公藤组中表达低于模型组(P<0.05)。结果证实,白喉乌头治疗类风湿关节炎的机制可能与其抑制肿瘤坏死因子α和白细胞介素1β表达有关。

BACKGROUND：The majority of rheumatoid arthritis treatment is chronic anti-arthritis drugs, biological agents and plant drugs. Among them, plant drugs have been widely concerned due to low cost and few adverse effects. OBJECTIVE：To observe the expression of tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β） in the synovium of col agen-induced arthritis rats, and explore the effect of Aconitum leucostomum Worosch. on the expression. METHODS：Forty-five rats were randomly divided into normal control group （8 rats） and model group （37 rats）. The col agen-induced arthritis model was established with the injection of type II bovine col agen into the end of the tail and paws. After the success of modeling, the 24 successful model rats were randomly selected and＆nbsp;divided into model group （8 rats）, Tripterygium wolfprdi polyglycoside group （8 rats） and Aconitum leucostomum Worosch. group （8 rats）. The arthritis index of the rats in the three intervention groups and one control group were evaluated weekly. After treated by intragastric administration for 4 weeks （Tripterygium wolfprdi polyglycoside group and Aconitum leucostomum Worosch. group were taken by the corresponding drug solution, model group and normal control group were taken by the same volume of physiological saline）, the expressions of TNF-αand IL-1βin the synovium were tested by immunohistochemistry. RESULTS AND CONCLUSION：Compared with the model group, the arthritis index of mice in Tripterygium wolfprdi polyglycoside group and Aconitum leucostomum Worosch. group was decreased significantly after treatment （P〈0.05）. The expression levels of TNF-αand IL-1βin the synovium of model group were significantly higher than those of the normal control group （P〈0.05）. After treatment with Aconitum leucostomum Worosch. and Tripterygium wolfprdi polyglycoside, the expression levels of TNF-αand IL-1βin the synovium was decreased compared with the model group （P〈0.05）. Experimental findings indicated that, the mechanism that Aconitum leucostomum Worosch. treats rheumatoid arthritis is related to the inhibition of TNF-αand IL-1β.