人参皂甙Rg1干预脊髓损伤模型大鼠转化生长因子β和脑源性神经营养因子的表达

Effect of Ginsenoside Rg1 on transformation growth factor-beta and brain-derived neurotrophic factor expression in spinal cord injury rats

背景:转化生长因子β和脑源性神经营养因子是脊髓损伤过程中的主要调节因子,研究转化生长因子β和脑源性神经营养因子在脊髓损伤过程中发生发展机制比较多,对于人参皂甙Rg1干预调节转化生长因子β和脑源性神经营养因子在脊髓损伤发作用的文献鲜有报道。目的:通过人参皂甙Rg1干预结合转化生长因子β和脑源性神经营养因子在分子蛋白水平上表达量的变化来研究人参皂甙在脊髓损伤后对脊髓及神经的保护作用。方法:将大鼠随机分为空白对照组、模型组和人参皂苷Rg1干预组。模型组和人参皂苷Rg1干预组大鼠以撞击法建立脊髓损伤模型。人参皂苷Rg1干预组大鼠在造模后24 h以10 mg/kg的人参皂甙Rg1腹腔注射,1次/d,连续14 d。空白对照组和模型组大鼠注射等体积生理盐水。结果与结论:与空白对照组相比,模型组和人参皂苷Rg1干预组大鼠血清中的丙二醛含量升高,超氧化物歧化酶的含量降低,脊髓组织中转化生长因子β表达水平增加,脑源性神经营养因子表达水平降低;与模型组相比,人参皂苷Rg1干预组大鼠血清中的丙二醛降低,超氧化物歧化酶的含量增加,脊髓组织中转化生长因子β表达水平降低,脑源性神经营养因子表达水平升高。提示人参皂苷Rg1对损伤的大鼠脊髓组织具有保护作用。

BACKGROUND：Transformation growth factor-β（TGF-β） and brain-derived neurotrophic factor （BDNF） are the main regulatory factors in the process of spinal cord injury. There are many researches for TGF-βand BDNF pathogenesis in the spinal cord injury, but the regulation of Ginsenoside Rg1 intervention on TGF-βand BDNF in the spinal cord injury is rarely reported. OBJECTIVE：To observe the effect of Ginsenoside Rg1 intervention on TGF-βand BDNF expression at themolecular protein levels, and to study the protection effect of Ginsenoside Rg1 on the spinal cord and nerve function after spinal cord injury. METHODS：Experimental rats were randomly divided into blank control group, model group and Ginsenoside Rg1 group. In the model and Ginsenoside Rg1 groups, spinal cord injury model was established with the impact method in rats. In the Ginsenoside Rg1 group, rats were intraperitoneal y injected with 10 mg/kg Ginsenoside Rg1 24 hours after modeling, once per day, for 14 days. Rats in the blank control and model groups were injected with equal saline. RESULTS AND CONCLUSION：Compared with the control group, serum malondialdehyde levels increased, the content of superoxide dismutase decreased, TGF-βexpression levels in spinal cord tissue increased, and BDNF expression levels decreased in the model and Ginsenoside Rg1 groups. Compared with the model group, serum malondialdehyde levels decreased, the content of superoxide dismutase increased, TGF-βexpression levels in spinal cord tissue decreased, and BDNF expression levels increased in the Ginsenoside Rg1 group. Ginsenoside Rg1 can protect the injury spinal cord in rats after spinal cord injury.