MicroRNA-182在胃癌中的表达及临床意义

Clinical significance of miRNA-182 in gastric cancer

目的:探讨微小RNA-182(miRNA-182)在胃癌中的表达及与临床病理特征和预后的关系。方法:收集50例胃癌患者肿瘤组织和非癌胃组织,采用RT-PCR方法检测miRNA-182的相对表达量,并分析其与临床病理资料和预后的相关性。同时,采用RT-PCR方法检测胃癌细胞株和正常胃上皮细胞中miRNA-182的相对表达量。结果:肿瘤组织中miR-182表达水平显著高于非癌胃组织(P<0.01),人胃癌细胞株中miR-182表达水平明显高于正常胃上皮细胞(P<0.01)。miR-182的表达水平与胃癌的大小、淋巴结转移、及临床分期呈明显相关(P<0.05)。miR-182低表达者生存时间明显高于高表达组,差异有显著统计学意义(P<0.05)。结论:miR-182在胃癌组织和胃癌细胞中表达均上调,且与病情进展和预后相关,可能成为判断胃癌预后的新的分子标记物。

Objective：To investigate the expression of miRNA - 182 in patients with gastric cancer and to analyze the association of the expression with the clinicopathological features and prognosis. Methods： The expression levels of miRNA - 182 in 50 tumor tissues and paired normal tissues of gastric cancer were detected by using RT - PCR. The expression of miRNA - 182 was also analyzed between gastric cancer cell lines and gastric epithelial cell lines. The correlations between the expression of miRNA - 182 and the pathological characteristics and prognosis were analyzed. Results：Expression levels of miRNA - 182 were up - regulated in tumor tissues as compared with the paired normal tissues （P 〈 0.05 ）. A similar pattern was also observed in gastric cancer cell lines. The expression of miRNA - 182 was associated with tumor size, lymphatic metastasis, and TNM stages （ P 〈 0.05 ）. The expression of miRNA - 182 was correlated with overall survival of gastric cancer （P 〈 0.05 ）. Conclusion ：The expression of miRNA - 182 was up - regulated in tumor tissues and cells, and may be a valuable parameter for prognostic evaluation of gastric cancer.