高效液相色谱-质谱法同时测定曲霉菌代谢物中的黄曲霉毒素及其同系物

Determination of aflatoxin and aflatoxin-like compounds in aspergilli metabolites by high performance liquid chromatography-mass spectrometry

建立了同时检测曲霉菌代谢物中黄曲霉毒素和同系物的超高效液相色谱-线性离子阱质谱测定方法。寄生曲霉（菌株3.124）经PDA液体培养基培养,Qu ECh ERS方法提取净化后经线性离子阱（QTrap）质量分析器分析（正离子模式,多反应检测）,检出3.124代谢物中黄曲霉毒素B1（AFB1）、黄曲霉毒素B2（AFB2）、黄曲霉毒素G1（AFG1）、黄曲霉毒素G2（AFG2）、O-甲基杂色曲霉素（MST）、杂色曲霉素（ST）6种真菌毒素。结果表明,6种代谢物在0.1~40μg/L范围内线型关系良好,相关系数均大于0.993,检出限在0.03~0.2μg/L之间,定量限在0.1~0.5μg/L之间。本方法 6种代谢物日内回收率为81.3%~92.1%,相对偏差（RSD）为4.3%~8.6%;日间回收率为81.8%~91.5%,RSD为4.0%~8.7%。方法可满足霉菌代谢物中黄曲霉毒素及其类似物的检测与确证的需要。

A method for simultaneous determination of aflatoxin and aflatoxin-like compounds in aspergilli metabolite was developed by high performance liquid chromatography-linear ion trap mass spectrometry. A. parasitieus（ strain 3. 124 ） was cultivated in PDA liquid medium, then the metabolites of strain 3. 124 was analyzed by mass analyzer of QTrap QuEChERS method. Six mycotoxins （multiple reaction monitoring （MRM））after extraction and purification by of AFB1 ,AFB2,AFG1 ,AFG2,MST and ST were found in the strain 3. 124 metabolites. The linear range of these six mycotoxins was 0. 1 - 40 μg/L with the correlation coefficients more than 0. 993. The limits of detection were in the range of 0. 03 - 0. 2 μg/L and the limits of quantification were in the range of 0. 1 - 0. 5 μg/L. The recoveries of inter-day ranged from 81.3% to 92. 1% with the relative standard deviations （RSDs） from 4. 3% to 8.6% while the recoveries of intra-day ranged from 81.8% to 91.5% with the relative standard deviations （RSDs） were from 4. 0% to 8.7%. This method is easy, sensitive and suitable for determination of aflatoxin and aflatoxin-like compounds in aspergilli metabolites.