牛Ⅱ型胶原诱导恒河猴关节炎的实验研究

Investigation of the arthritis induced by bovine type Ⅱ collagen in rhesus monkeys

目的观察牛Ⅱ型胶原诱导川西恒河猴关节炎后,相关炎症指标与关节X光影像学的变化。方法将6只恒河猴分别sc 1.5 mg牛Ⅱ型胶原诱导关节炎,检测恒河猴的体重、体温、血液学及血生化指标,观察恒河猴的症状并每天进行临床相关性评分。免疫后第4周与第8周分别进行四肢关节的X光影像学分析。结果 6只恒河猴中有1只属于急性活动期反应(免疫后第1周CRP>50 mg·L-1),3只属于慢性活动期反应(免疫后第4周开始CRP>50 mg·L-1);注射胶原后猴的体重增长率<14%或呈负增长,全身临床评分持续增长;与免疫前比较,中性粒细胞(NEUT)、单核细胞(MONO)计数升高,白蛋白(ALB)与血红蛋白(HGB)降低(P<0.05);X光平片显示第8周与第4周相比有进展,与关节病理学检查结果相符。结论早期急性活动期反应者与慢性活动期反应者的共同特点是NEUT、MONO计数升高,体重、ALB与HGB降低或增长缓慢;CRP可以作为早期关节炎发病的指标,X线影像学显示关节病变程度加重,与临床类风湿关节炎的症状类似并有其特殊表现。

OBJECTIVE To determine the clinical indexes of rhesus monkeys which were immunized by bovine type Ⅱ collagen.METHODS Six monkeys were injected subcutaneously with 1. 5 mg per animal by bovine type Ⅱ collagen. Body weight,body temperature and biochemistry markers were detected,and the symptoms of each monkey were scored every day. Four limbs of each monkey were detected by X-ray after the 4th weeks and 8th weeks. RESULTS One in six monkeys was acute phase response animal（ CRP 50 mg·L-1,the first week）. 3 monkey were late mild responders（ CRP 50 mg·L-1,the fourth week）. After the immunization,the body weight was less 14% than the first week or showed negative growth,systemic clinical scores of animals continued to grow.Neutrophils（ NEUT）,monocytes（ MONO） increased compared with pre-immune point,albumin（ ALB） and hemoglobin（ HGB）decreased（ P 0. 05）. X-ray showed that the joints damage progressed,and it was consistent with the result of arthropathology examination. CONCLUSION Acute phase responder and mild phase responders showed the similarities with clinical manifestations,they could provide a useful tool for pharmacodynamics researches.