纳米银凝胶经阴道给药后在家兔体内的分布特征

Distribution of silver in the rabbits after vaginal delivery of nano silver gel

目的给予家兔阴道内纳米银凝胶后,研究银在家兔体内的分布情况。方法单剂量和多剂量经♀家兔阴道给予纳米银凝胶200 mg·kg-1后,于不同时间点处死家兔并剖取主要组织器官,用ICP-MS法测定给药前、后家兔各器官中银的含量。结果与0 h组比较,单剂量给药后,阴道、子宫和肝脏中银的含量明显增加,其余组织中未见明显分布,给药72 h后各组织中银的含量均恢复至本底水平;多剂量给药后,末次给药2~24 h内阴道、子宫、肝脏、脾、肾、大肠和小肠中有银的分布,心、脑、肺和胃未见明显分布,末次给药72 h后各组织中银的含量均恢复至本底水平。结论单剂量和多剂量经家兔阴道给予纳米银凝胶后,给药2~24 h内银在组织中有分布,给药72 h后各组织中均未见明显银蓄积。

OBJECTIVE To investigate the distribution of silver in the female rabbits after vaginal delivery of nano silver gel.METHODS Female rabbits were vaginally treated with nano silver gel at 200 mg·kg-1. Then they were sacrificed at the different time points and major tissues were collected. The contents of silver in the tissues were determined by ICP-MS before and after delivery.RESULTS Compared with the blank control,the amounts of Ag in vagina,uterus and liver were obviously increased in rabbits treated with silver gel via vaginal once. No distribution was observed in the other tissues after the single treatment. However,the significant distribution of Ag after multiple treatments hasn＇t been found in the heart,brain,lung and stomach. The amounts of Ag returned to the baseline at 72 h after single and multiple treatments. CONCLUSION Distribution of Ag in the rabbits observed in 2-24 h aftersingle and multiple treatments. No significant accumulation has been found at 72 h after treatment.