2013年浙江省南美白对虾虾苗病毒携带情况分析

Analysis on presence of virus in Penaeus vannamei shrimp larvae in Zhejiang Province in 2013

病毒病频发是导致对虾养殖失败的最重要原因之一,为摸清浙江省南美白对虾虾苗病毒携带情况(白斑病毒、桃拉病毒、传染性皮下及造血组织坏死病毒),进一步提高水产苗种质量安全水平,于2013年对浙江省主要的南美白对虾虾苗场进行了病毒携带情况调查,并结合虾苗来源进行分析。检测方法采用国标、行标中的PCR或RT-PCR方法。结果显示,113份南美白对虾苗种来源均为外省,其中福建省58批次,广东省、海南省各26批次,其他地区3批次,共有68份检出病毒,总阳性率为60.18%,其中传染性皮下及造血组织坏死病毒(IHHNV)阳性59份,占52.21%;白斑病毒(WSSV)阳性18份,占15.93%;桃拉病毒(TSV)阳性1份,占0.88%,WSSV和IHHNV同时阳性的10份,占8.85%。

Outbreak of virus diseases is one of the critical reason for breeding failure of shrimp. In order to clarify the presence of virus disease in shrimp larvae of Penaeus vannamei in Zhejiang Province, the detection of main virus （white spot syndrome virus, WSSV, Taura syndrome virus, TSV, and infectious hypodermal and hematopoietic necrosis virus, IHHNV） in shrimp larvae was investigated. Totally, 113 samples were collected and detected by PCR and RT-PCR method, among which 58 samples were from Fujian Province, 26 samples were from Guangdong Province, 26 samples were from Hainan Province and 3 samples were from other provinces. The results showed that the total virus positive rate was 60. 18% , among which the virus positive rates of IHHNV, WSSV TSV, and IHHNV ＆WSSV were 52. 21%, 15.93%, 0. 88% , 8.85% , respectively.