成年大鼠骨髓间充质干细胞复合组织工程人工神经修复1cm坐骨神经缺损

Repairment of 1 cm sciatic nerve defect by bone marrow mesenchymal stem cell of adult rat combined with tissue-engineered artificial nerve

目的探讨骨髓间充质干细胞(BMSC)作为组织工程化人工神经的种子细胞移植治疗外周神经损伤的效果。方法从成年大鼠的骨髓中分离培养得到BMSC,复合去细胞神经支架构建"组织工程化人工神经"。移植后分为BMSC+去细胞SD大鼠神经导管组(BMSC治疗组)和空细胞SD大鼠神经导管组(阴性对照组),每组各5只。比较两组大鼠术后2、4、8周损伤侧坐骨神经功能指数(SFI),术后8周损伤侧坐骨神经传导功能和小腿三头肌湿重恢复率等修复效果的指标。结果BMSC治疗组的坐骨神经修复术后2、4、8周损伤侧坐骨神经功能指数,术后8周损伤侧坐骨神经传导功能和小腿三头肌湿重恢复率均优于阴性对照组(均为P<0.05)。结论 BMSC复合去细胞神经支架的组织工程化人工神经可有效促进神经再生和功能恢复。

Objective To discuss the effect of bone marrow mesenchymal stem cell （BMSC）as the seed cell transplantation of tissue-engineered artificial nerve in the treatment of peripheral nerve injury. Methods BMSC was obtained from the bone marrow of adult rat through isolation and culture and combined with acellular nerve scaffold to construct ‘tissue-engineered artificial nerve’.After transplantation,rats were divided into two groups,the BMSC ＋acellular nerve conduit group（BMSC treatment group）and the empty cell conduit group（negative control group）with 5 rats in each group.Sciatic functional index （SFI）of the affected side of rats was compared between two groups at 2 weeks,4 weeks and 8 weeks after the surgery.Moreover,the sciatic conduction,recovery rate of tricipital muscle wet weight and other repair effects of the affected side were compared between two groups at 8 weeks after the surgery.Results The indicators of BMSC treatment group, including SFI assessed at 2 weeks,4 weeks and 8 weeks after the surgery as well as the sciatic conduction and recovery rate of tricipital muscle wet weight assessed at 8 weeks after the surgery,were better than those of the negative control group（all in P 〈0.05）.Conclusions BMSC combined with tissue-engineered artificial nerve of acellular nerve scaffold can effectively promote nerve regeneration and function recovery.