3种癫痫模型的制备及应用分析

Analysis of Preparation and Application of 3 Epilepsy Models

目的:探讨不同癫痫模型的制备及其在癫痫研究方面的应用。方法:选择成年雄性SD大鼠60只随机分为杏仁核慢点燃组、杏仁核快点燃组、注射匹罗卡品组,每组20只动物,观察各组大鼠的点燃率,模型大鼠癫痫发作级别及癫痫行为Racinel评分,同时观察模型大鼠癫痫发作时的脑电图。结果:成功获得各组癫痫模型动物,各组Racinel评分差异有统计学意义(P<0.05),其中慢点燃组动物持续时间较长,快点燃组出现典型癫痫行为较早,匹罗卡品组动物存活时间长。结论:与杏仁核电点燃模型相比,匹罗卡品点燃癫痫动物成功率高,癫痫行为保持时间较久,能更好的为临床所应用,但匹罗卡品所致癫痫大鼠痫性发作持续时间变异较大,这可能为临床筛药带来了一定的不便。

Objective： To investigate the preparation of different epilepsy models and its application in epilepsy research. Methods： Sixty adult male SD rats were randomly divided into amygdala kindling slow group,amygdala kindling fast group,and pilocarpine injection group with 20 rats in each. Observing seizure grade of epilepsy rats and epileptic behavior Racinel scores,and EEG of rats during seizure. Results： Various epilepsy animal models were successfully obtained,Racinel scores were significantly different among each group（ P〈0. 05）. In which,epilepsy of slowly burning animals continued for a longer time,quick burning group showed typical epileptic seizure behavior earlier,and the pilocarpine animals lived longer time. Conclusion： The pilocarpine injection group showed higher success rate and epilepsy behavior lasted longer,which can better serve clinical purpose. However,pilocarpine triggered epileptic seizure time varies greatly,which might cause certain inconvenience for clinical screening.