PGC-1α对人血管内皮细胞VEGF表达及管腔形成的调控

Regulation of VEGF expression and tube formation in human vascular endothelial cells by PGC-1α

目的 探讨过氧化物酶增殖激活受体-γ共激活子-1α（PGC-1α）对人血管内皮细胞（HUVEC）血管内皮生长因子（VEGF）表达及管腔形成的影响.方法 实验研究.HUVEC加入重组PGC-1α蛋白作为重组组,未加入重组蛋白的细胞作为对照组.加入重组蛋白24h后,将2组细胞分别置于常氧（20％ O2）和低氧（1％O2）环境下继续培养16 h,采用real-time PCR和ELISA分别检测VEGF mRNA和蛋白的表达变化.加入重组蛋白48 h后,将2组细胞转移至Matrigel基底膜包被的培养板,并分别置于常氧和低氧环境下继续培养48 h,采用光学显微镜观察细胞在体外管腔形成的情况.应用单因素方差分析对实验数据进行处理.结果 real-time PCR和ELISA检测发现常氧和低氧环境下细胞加入重组PGC-1α蛋白后VEGF mRNA和蛋白的表达均较对照组上调,差异有统计学意义（F=131.67、65.96,P＜0.01）.2种环境下,加入重组PGC-1α蛋白后细胞管腔形成能力均较对照组增强.结论 PGC-1α能上调HUVEC VEGF的表达,并促进细胞管腔的形成.

Objective To investigate the effect of peroxisome-proliferator-activated receptor-γ coactivator-1α （PGC-1α） on vascular endothelial growth factor （VEGF） expression and tube formation in human vascular endothelial cells.Methods This was an experimental study.Recombinant PGC-hα protein was added to human vascular endothelial cells （HUVEC）,and no recombinant PGC-1o protein was added to HUVEC for the control group.After 24 hours of incubation,the two groups of cells were placed into a normoxic （20% O2） or hypoxic （1% O2） environment for another 16 hours.The expression of VEGF mRNA and protein were detected by real-time PCR and ELISA.After 48 hours of incubation,the two groups of cells were plated onto Matrigel-coated plates and cuhured under normoxic or hypoxic conditions for another 48 hours.The tube formation of cells was observed by optical microscopy.Experimental data were analyzed by one-way ANOVA.Results VEGF mRNA and protein levels in the cells were significantly increased by recombinant PGC-1α protein both under normoxia and hypoxia conditions compared to the control groups （F=131.67,65.96,P〈0.01）.The tube formation ability of cells was enhanced significantly after treatment with recombinant PGC-1α protein compared to the control groups under both normoxia and hypoxia conditions.Conclusion PGC-lα can upregulate the expression of VEGF and promote tube formation in HUVEC.