乳杆菌吸附塑化剂的影响因素分析及效果评价

Influencing factors analysis and effect evaluation of Lactobacillus strains to bind plasticizers

【目的】评价嗜酸乳杆菌(Lactobacillus acidophilus)NCFM和类食品乳杆菌(Lactobacillus paralimentarius)412吸附塑化剂的效果,初步探讨影响其吸附的因素。【方法】基于3种邻苯二甲酸酯类塑化剂:邻苯二甲酸二乙酯(DEP)、邻苯二甲酸二丁酯(DBP)和邻苯二甲酸二乙基己酯(DEHP)的HPLC检测方法,评价了菌株NCFM和412与塑化剂共同温育时的吸附状况,优化菌株吸附DBP的条件,研究菌株在热、酸及Na Cl处理后吸附塑化剂的稳定性。【结果】乳杆菌NCFM和412对3种邻苯二甲酸酯类塑化剂均有不同程度的吸附效果,其中菌株NCFM对3种塑化剂DEP、DBP和DEHP的吸附率分别为21.48%、43.32%和9.62%,吸附效果优于菌株412,其中DBP的吸附效果最好。在温度为37?C时,菌株NCFM吸附DBP的最佳时间是4 h。热、酸和Na Cl处理都会显著提高菌体NCFM吸附塑化剂的效果。【结论】嗜酸乳杆菌NCFM通过吸附作用具有清除3种邻苯二甲酸酯类塑化剂的效果,可以作为潜在的塑化剂生物脱除剂使用。

[Objective] This study was designed to investigate the binding of two Lactobacillus strains, i.e., L. acidophilus NCFM and L. paralimentarius 412, to plasticizers. Several factors which affect the ability of the bacteria to bind plasticizers were also detected. [Methods] HPLC was used to detect the binding efficiency of two lactobacillus strains to three phthalates plasticizers, namely DEP, DBP and DEHP, respectively. The adsorption conditions of two strains to DBP were optimized when the bacterial cells were subjected to heat, acid or NaCl treatment. [Results] Two Lactobacillus strains showed an adsorption to three phthalic acid esters plasticizers, but strain NCFM was better in binding plasticizers than strain 412, especially in adsorbing the plasticizer DBP. The binding percentages of strain NCFM to three phthalates DEP, DBP and DEHP was 21.48%, 43.32% and 9.62%, respectively.Strain NCFM bound the maximal DBP after incubation for 4 h at 37 ℃. The efficiency of strain NCFM plasticizers-adsorbing was significantly improved via heat, acid and NaCI treatment. [Conclusion] L. acidophilus NCFM showed good ability to bind phthalic acid esters plasticizers. Strain NCFM should be a candidate as one of biological tools to remove plasticizers from the environments linked to food products.