基于超弱延迟发光的小麦生活力检测方法研究

Detection for Viability of Wheat Based on Ultra- Weak Delayed Bioluminescence

以郑麦9023为试验对象,研究了不同生活力和不同测试条件下小麦样品超弱延迟发光的变化情况。通过人工快速陈化的方法获得不同生活力的小麦样品,对这些小麦样品的延迟发光特性进行系统研究,在此基础上以温度为例研究了测试条件对小麦延迟发光的影响。结果表明,同一品种不同生活力小麦样品的延迟发光能力差异明显,小麦的延迟发光强度与其生活力显著负相关,生活力越强,其延迟发光能力越弱;小麦的延迟发光能力受其自身温度的影响显著,随着温度的升高,其延迟发光能力减弱。本研究发现,利用超弱延迟发光进行小麦生活力检测是可行的,但需要对测试条件进行严格控制。

The variation of the ultra-weak delayed bioluminescence under different viability and different test conditions has been investigated with zhengmai 9023 as an experimental object. The different viability wheat sample can be obtained by rapid artificial aging. The characteristic of the delayed bioluminescence from different viability and the influence of temperature on the delayed bioluminescence are studied. The results show that the difference of the delayed bioluminescence ability for different viability with the same seeds is obvious. As the aging degree of wheat rises, the ability of ultra-weak delayed bioluminescence increases correspondingly. There exists the correlation between the delayed bioluminescence and its viability. The influence of temperature on the delayed bioluminescence is obvious. As the temperature rises, the intensity of ultra-weak photon emission decreases gradually. The research demonstrates that it is feasible to detect wheat viability based on the ultra-weak delayed bioluminescence under the strict control of test conditions.