摘要
目的:观察雷公藤内酯对海人活化的BV-2小胶质细胞Ras、Raf蛋白及mRNA表达的影响,揭示雷公藤内酯抑制海人酸活化的BV-2小胶质细胞增殖的分子机制。方法:免疫组化法检测BV-2小胶质细胞Ras和Raf蛋白表达,RT-PCR法检测BV-2小胶质细胞Ras和Raf mRNA表达。结果:免疫组化结果显示,海人酸组BV-2小胶质细胞Ras和Raf蛋白表达与对照组相比显著增加(P<0.05),雷公藤内酯组BV-2小胶质细胞Ras和Raf蛋白表达与海人酸组相比显著降低(P<0.05)。RT-PCR结果显示,海人酸组BV-2小胶质细胞Ras和Raf mRNA表达水平与对照组相比显著增加(P<0.05),雷公藤内酯组BV-2小胶质细胞Ras和Raf mRNA表达水平与海人酸组相比显著降低(P<0.05)。结论:雷公藤内酯可能通过下调BV-2小胶质细胞Ras、Raf蛋白及mRNA表达抑制海人酸活化的BV-2小胶质细胞增殖。
Objective：To observe the effect of triptolide on the expressions of Ras and Raf in kainic acid-acti-vated BV-2 microglial cells and reveal the molecular mechanism of triptolide for inhibiting the proliferation of BV-2 microglial cells.Methods：Immunohistochemistry technique was uesd to detect the expressions of Ras and Raf protein in BV-2 microglial cells.RT-PCR was used to detect the expressions of Ras and Raf mR-NA in BV-2 microglial cells.Results： Immunohistochemistry technique showed that the expressions of Ras and Raf protein in BV-2 microglial cells in kainic acid group increased significantly compared with those in the control group （P〈0.05）.However, the expressions of Ras and Raf protein in BV-2 microglial cells in triptolid group decreased significantly compared with those in the kainic acid group （P〈0.05）.The results of RT-PCR showed that the expressions of Ras and Raf mRNA in BV-2 microglial cells in kainic acid group increased significantly compared with those in the control group （P〈0.05）.However, the expressions of Ras and Raf mRNA in BV-2 microglial cells in triptolid group decreased significantly compared with those in the kainic acid group （ P〈0 .05 ） .Conclusion：Triptolide can suppress the proliferation of kainic acid-activated BV-2 microglial cells by downregulating the expressions of Ras and Raf protein and mRNA.
出处
《中医药信息》
2015年第3期23-26,共4页
Information on Traditional Chinese Medicine
基金
辽宁省科学技术计划项目(No.2013225086)
