高脂诱导营养性肥胖大鼠模型红细胞膜生物学研究

Biological Study on the Components of Erythrocyte Membrane of Nutritional Obese Rats by High Fat Diet

目的:探讨高脂诱导营养性肥胖大鼠中医证候属性,并分析该模型在红细胞膜上的生物学差异。方法:SD大鼠71只,随机分为普食组(10只)和高脂组(61只),分别喂饲普通饲料和高脂饲料,连续喂养9周。模型复制成功后,观察大鼠表观特征;取模型成功大鼠10只进行体质量、Lee's指数、脂体比检测;取血测定甘油三酯(TG)、胆固醇(CHO)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)的水平;测定全血黏度、血浆黏度以及红细胞膜组分相关指标,并与普食组进行比较。结果:该种造模方法成模率为82%,且该模型具有中医痰湿证的表观特征;与普食组比较,高脂组大鼠体质量以及脂体比较具有极显著性差异(P均<0.01),Lee's指数显著升高(P<0.05);甘油三酯(TG)含量显著升高(P<0.05),高密度脂蛋白(HDL-C)含量显著降低(P<0.05),全血黏度在200s-1切变率以及30s-1切变率下,高脂组显著升高(P<0.05),血浆黏度显著升高(P<0.05),红细胞膜上Na+-K+-ATP酶活力、超氧化物歧化酶(SOD)活力均极显著降低(P<0.01),丙二醛(MDA)含量显著升高(P<0.05)。结论:高脂诱导营养性肥胖大鼠模型的中医证候属性可为痰湿证,该模型可能由于Na+-K+-ATP酶及SOD活力的降低,MDA含量的升高导致全血黏度发生改变。

Objective：To investigate TCM symptoms properties of the nutritional obese rats by high fat diet ,and to analyze biological difference of the changes of the components of erythrocute membrane.Methods：Seventy-one Wistar rats were randomly divided into two groups, ordinary feeding group and high fat feeding group, with 10 rats in ordinary feeding group and 61 rats in high fat feeding group.The control group was fed with nor-mal diet,and the model group was fed with nurtritional diet, continuing to feed for 9 weeks.Observe apparent chracteristics in rats after success of the model replication.The body weight, Lee＆#39;s index, body fat/body weight were measured;the degrees of TG,CHO,HDL-C and LDL-C were detected after blood withdrawing. The whole blood viscosity,the plasma viscosity and the components of erythrocyte membrane were also deter-mined and compared with those in the control group.Results：The successful rate of modeling method was 82%, and the models had apparent characteristics of phlegm dampness syndromes of traditional Chinese medi-cine.Compared with the ordinary feeding group, the weight and body fat/body weight of model group were in-creased significantly（P〈0.01,P〈0.01）; the Lee＆#39;s index was also increased （P〈0.05）.TG levels in-creased markedly（P〈0.05）.HDL-C levels decreased markedly（P〈0.05）.The whole blood viscosity was increased under 200s-1 and 30s-1（P〈0.05,P〈0.05）.The plasma viscosity and the contents of MDA in the model group were increased （P〈0.05,P〈0.05）, SOD and Na＋-K＋-ATP enzyme activity of erythro-cyte membrane were decreased significantly（P〈0.01,P〈0.01）.Conclusion：The TCM symptoms properties of the nutritional obese rats by high fat diet are Phlegm dampness syndromes.The model can decrease SOD and Na＋-K＋-ATP enzyme activity and increase the degree of MDA of erythrocyte membrane which may be one of the mechanisms of improving the blood viecosity.