电针曲池、神门、足三里穴对胸主动脉缩窄法诱导心肌肥厚大鼠Bcl-2及Bax的影响

Effect of Electroacupuncture at Quchi,Shenmen and Zusanli on Bcl-2 and Bax in Rats with Cardiac Hypertrophy Induced by Abdominal Aortic Banding

目的:探讨电针曲池穴、神门穴、足三里穴对心肌肥厚模型大鼠Bcl-2及Bax影响。方法:50只Wistar大鼠随机分为模型组、假手术组、曲池+足三里组、神门+足三里组、曲池+神门+足三里组,每组10只,胸主动脉缩窄法建立大鼠心肌肥厚模型,电针组连续治疗14天,通过实时定量PCR及Western blot测定心肌组织中Bcl-2、Bax mRNA及蛋白的表达水平。结果:曲池+足三里组、神门+足三里组、曲池+神门+足三里组Bcl-2及Bax mRNA及蛋白的表达水平在治疗后与模型组相比,有显著性差异(P<0.05)。与模型组对比各电针组血流动力学均有明显改善。结论:同时电针曲池穴+神门穴+足三里穴可明显抑制胸主动脉缩窄法所引起的心肌肥厚,其机制可能是通过改善心脏血流动力学、以及促进Bcl-2的表达同时抑制Bax的表达进而抑制心肌细胞的凋亡,从而产生延缓及抑制心肌肥厚进展的作用。

Objective：To discuss the effect of electroacupuncture at Quchi, Shenmen and Zusanli on Bcl-2 and Bax in rats with cardiac hypertrophy.Methods：Fifty Wistar rats were randomly divided into five groups （ a model group,a sham operation group,a Quchi ＆amp; Zusanli group,a Shenmen ＆amp; Zusanli group and a Quchi ＆amp;Shenmen ＆amp;Zusanli group） , including ten rats in each group.The rat models with cardiac hypertrophy were prepared and rats in electroacupuncture groups received a continuous wave for the treatment of 2 weeks. Through the method of real time PCR and western blot, the expression levels of Bcl-2 and Bax mRNA and protein in heart tissue were detected.Results：After treatment,the expression levels of Bcl-2 and Bax mRNA and protein in heart tissue were significantly different in Quchi ＆amp; Zusanli group, Shenmen ＆amp; Zusanli group and Quchi ＆amp; Shenmen＆amp;Zusanli group compared with model group（P〈0.05）.The hemodynamics of elec-troacupuncture groups improved significantly compared with model group.Conclusion： Electroacupuncture at Quchi ＆amp;Shenmen＆amp;Zusanli acupoints can prevent the myocardial hypertrophy.The mechanism may be by im-proving cardiac hemodynamics and promoting the expression of Bcl-2 and inhibiting the expression of Bax to inhibit the apoptosis of myocardial cells,then playing a role in delaying and inhibiting the process of myocardi-al hypertrophy.