CyPA 及 CD147在动脉粥样硬化兔血管重构中的表达

Expressions of Cy PA and CD147 in vascular remodeling of atherosclerosis rabbits

目的探讨亲环素A(Cy PA)/CD147在动脉粥样硬化(AS)兔血管重构中的表达特点。方法新西兰雄性白兔32只(3月龄),随机分为对照组、模型组、液氮冻伤组、高脂饮食组(每组8只)。0周和13周分别检测兔血清CD147和Cy PA表达水平。术后13周处死动物,取血管石蜡包埋切片,行HE、弹力纤维染色,观察血管形态,并做巨噬细胞、Cy PA、CD147免疫组织化学染色。利用计算机图像分析系统观察动脉损伤后血管重构相关指标的变化。结果 13周时模型组、液氮冻伤组、高脂饮食组血Cy PA与CD147水平较同时期对照组均明显升高(P<0.05),尤以模型组为甚。除对照组,其余3组均可见内膜增生,模型组还可见典型AS斑块形成;斑块内及增生内膜均可见Cy PA及CD147的表达。模型组、液氮冻伤组、高脂饮食组最小管腔直径及管腔面积较对照组均明显减小(P<0.05),内膜面积、中膜面积、最大内膜厚度、管腔狭窄度较对照组明显增大(P<0.05)。结论 Cy PA与CD147在AS兔血管重构中的表达明显升高。

Objective To explore the expressions of Cyclophilin A（ CyPA）/ CD147 in vascular remodeling of ath-erosclerosis（AS）rabbits. Methods 32 healthy male New Zealand rabbits（3 months）were randomly divided into the control group,model group,cold-induced injury group,hyperlipid group（8 in each group）. Expressions of CyPA and CD147 in serum of rabbits were tested at the beginning and the 13th week,respectively. All rabbits were sacrificed after 13 weeks. Then paraffin-embedded sections were used for HE staining and special staining for elas-tic fiber to analyze the changes of vascular morphology. Additionally,macrophagocyte / CyPA/ CD147 monoclonal antibodies were applied to immunohistochemistical staining and the changes of related vascular remodeling indexes after arterial injury were observed using computerized image analyzing system. Results The expressions of CyPA and CD147 in serum increased significantly in the model group,cold-induced injury group and hyperlipid group compared with those in control group at 13th week（P 〈 0. 05）,respectively,especially the model group. Hyper-plastic intima was found in the model group,cold-induced injury group and hyperlipid group,but not in the control group. Additionally,atherosclerotic plaque could be found in the model group. There were high expressions of CyPA and CD147 in atherosclerotic plaques and hyperplastic intima in the model group,also in the cold-induced in-jury group and hyperlipid group. The minimum lumen diameter and lumen area significantly decreased in these three group compared with the control group（P 〈 0. 05）. However,the intima area,media area,maximum intima thickness and lumen stenosis increased significantly（ P 〈 0. 05）. Conclusion High expressions of CyPA and CD147 are found in Remodeling Vascular of atherosclerosis rabbits.