摘要
目的研究牛磺熊去氧胆酸(TUDCA)对肺纤维化防治作用。方法建立转化生长因子-β1(TGF-β1)诱导的人胚肺成纤维细胞(MRC-5)肺纤维化细胞模型。用MTS法测定TUDCA的安全实验剂量后,用实时定量-PCR和Western blot检测α-平滑肌动蛋白(α-SMA)和纤连蛋白的mRNA和蛋白表达。结果 1)TUDCA对MRC-5细胞无明显细胞毒作用。2)TUDCA显著缓解TGF-β1诱导的α-SMA和纤连蛋白的表达。结论 TUDCA可以缓解TGF-β1诱导的肺纤维化表型。
Objective To investigate the prophylactic and therapeutic effects of tauroursodeoxycholic acid （TUDCA） on transforming growth factor-β1 （TGF-β1）-induced fibrosis in MRC-5 cells.Methods Establish TGF-β1-induced MRC-5 pulmonary fibrosis model.Determine cytotoxicity of TUDCA on MRC-5 cells by MTS.Realtime PCR and Western Blot were applied to quantify the expression of fibrosis biomarker α-SMA and fibronectin.Results 1） TUDCA was well tolerated by MRC-5 cells.2） TUDCA significantly alleviates the expression of α-SMA and fibronectin induced by TGF-β1 in MRC-5 cells.Conclusions TUDCA alleviates TGF-β1-induced pulmonary fibrotic phenotype of MRC-5 cells.
出处
《基础医学与临床》
CSCD
2015年第6期767-771,共5页
Basic and Clinical Medicine
基金
公益性行业科研专项(201302017)
协和青年基金资助
中央高校基本科研业务费专项资金(3332013132)
