乙醇浴“一步交联”法制备明胶三维大孔纳米纤维支架

3D Macroporous Nanofibrous Scaffolds of Gelatin Made by One-step Crosslink in Ethanol Bath

目的:探讨采用乙醇浴"一步交联"法制备静电纺丝水溶性大分子纤维支架。方法:分别采用乙醇浴"一步交联"法和"二步交联"法制备明胶静电纺丝纤维,分析两种方法对纤维形貌、结构、交联度及稳定性的影响。结果:与"二步交联"纤维相比,"一步交联"法收集的明胶纤维直径更细,孔隙更大,吸水率更高,能维持纤维及支架的结构。通过对交联剂浓度及交联时间进行调整,可以改变纤维的交联度。"一步交联"纤维已具有良好的稳定性,无需再进行交联处理。结论:乙醇浴"一步交联"技术能有效维持明胶纤维的形貌及结构,提高交联效率,制备出较理想的三维大孔纳米支架。

Objective：To prepare electrospinning nanofibrous scaffolds of water-soluble polymers with one-step crosslink technique in ethanol bath.Methods：The fibers received by the ethanol bath were prepared by the technique of one-step crosslink or two-step crosslink.The effect of different crosslink methods on the morphology,structure,crosslink degree,and stability of the fibers was analyzed.Results：Compared with the fibers prepared by the two-step crosslink,the gelatin fibers of one-step crosslink had thinner fiber diameters,larger pores,and higher water up-take,which could sustain the structure of the fibers and scaffolds.The degree of the crosslink could be changed by adjusting the concentration of the crosslinker and the time of crosslinking.The fibers of one-step crosslink were stable enough that additional crosslink treatment was not needed.Conclusion：The technique of onestep crosslink using ethanol bath could sustain the morphology and structure of the fibers,improve the efficiency of crosslink,and prepare the 3D macroporous nanofibrous scaffolds.