尿中总溴测定的电感耦合等离子质谱法

Determination of total Bromine in urine by inductively coupled plasma mass spectrometry （ICP-MS）

目的 探讨1-溴丙烷职业接触人群尿中总溴的测定方法.方法 经1％稀硝酸稀释后的尿样直接进入电感耦合等离子质谱仪（ICP-MS）中检测,标准曲线定量.结果 尿中总溴含量的线性范围为0.005～1.000 mg/L,线性相关系数r＞0.999;当添加浓度为0.020、0.050和0.150 mg/L时回收率为95％～98％,批内精密度为1.4％～3.2％,连续3天测定批间精密度为3.4％～5.0％;尿中总溴可在-20℃冰箱中放置3个月以上不损失.结论 使用ICP-MS方法测定尿中总溴具有快速,准确,线性范围宽的特点,方法各项指标均符合GBZ/T 210.5-2008《职业卫生标准制定指南第5部分：生物材料中化学物质测定方法》的要求,适于推广应用.

Objective To establish a method to determine total bromine in urine.Method Diluted urine samples were directly introduced into ICP-MS then quantized by standard curve.Result Total bromine in urine was linear within 1.0～50 mg/L with r＞0.999,When spiked at a concentration of 0.020 mg/L,0.050 mg/L,0.150 mg/L,the recovery was 95％～98％,intra-assay precision was 1.4％ 3.2％,inter-assay precision was 3.4％ to 5.0％.Urine could store in-20 ℃ refrigerator 3 months without any bromine loss.Conclusion Using ICP-MS to determine the urinary total bromine,the method is fast,accurate,wide linear range of features,could meet with the requirement of Part 5 of occupational health standards guide：Method determination of chemical substances in biological materials （GBZ/T 210.5-2008）,a strong competitive advantage in a wide range of survey,suitable for promotion.