黑龙江地区耐碳青霉烯类鲍曼不动杆菌表型检测及同源性分析

Phenotype test and homology analysis of carbopenems antibiotics resistance acinetobacter baumannii in Heilongjiang province

目的对黑龙江地区耐碳青霉烯类抗生素鲍曼不动杆菌(carbopenems antibiotics resistance acinetobacter baumannii,CRAB)表型检测及同源性分析,了解该地区耐碳青霉烯类鲍曼不动杆菌的耐药机制及耐药菌株的克隆动向。方法采用改良Hodge试验,多底物-多抑制剂协同法分类检测碳青霉烯酶(carbapenem-hydrolyzingβ-lactamases,CHBLs);应用PCR扩增OXA-23、OXA-24、OXA-58、OXA-51等对收集的菌株进行分子分型,分析其耐药菌株之间的亲缘性关系。结果 319株CRAB的临床分离株中,286株改良Hodge试验阳性即产碳青霉烯酶,其中187株CRAB产丝氨酸类碳青霉烯酶(SCHBLs),无金属类碳青霉烯酶(MCHBLs)。187株CRAB碳青霉烯类酶基因扩增显示OXA-51为阳性扩增,OXA-23基因阳性率为98%,OXA-24和OXA-58基因未扩增出特异条带。结论 OXA-23基因是在黑龙江地区流行的CRAB主要基因型。

Objective To understand the resistance mechanism and epidemic resistance clone of carbopenems antibiotics resistance acinetobacter baumannii （ CRAB ） in Heilongjiang prov-ince by the phenotype test and homology analysis .Methods Modified Hodge test , multi sub-strate multi inhibitor synergy classification detecting carbapenemase （ CHBLs） and PCR ampli-fication of OXA-23 , OXA-24 , OXA-58 , OXA-51 were used to analyze the molecular type and the genetic relationship between resistant strains .Rseults Two hundred and eighty-six strains of the 319 clinical CRAB were positive in modified Hodge test .All the 187 strains produced serine carbapenemase （SCHBLs） with no metal carbapenemase （MCHBLs） found.OXA-51 gene were positive among the 187 CRAB strains , and the positive rate of OXA-23 gene was 98%.None specific bands of OXA-24 and OXA-58 gene were found .Conclusion OXA-23 is the major gene of CRAB prevalent in Heilongjiang area .