自噬基因LC3在卵巢子宫内膜异位症中的表达及意义

Expression of autophagy gene LC3 in ovarian endometriosis and its significance

目的：研究自噬基因LC3在卵巢子宫内膜异位症患者中的表达情况，探讨其与卵巢子宫内膜异位症发生、发展的相关性及意义。方法用Real-time PCR方法，对正常子宫内膜组织以及卵巢子宫内膜异位症患者的在位内膜组织、异位内膜组织进行LC3 mRNA定量分析，探讨其与临床分期的关系。结果 LC3 mRNA在卵巢子宫内膜异位症患者在位内膜组、异位内膜组和正常子宫内膜组中的表达存在显著差异（ P＜0.05）。异位内膜组表达显著低于正常内膜组，异位内膜组与在位内膜组相比表达明显下调（ P＜0.05） ，在位内膜组和正常内膜组间比较无明显差异（ P＞0.05）。 LC3 mRNA 在卵巢子宫内膜异位症不同临床分期中，异位内膜组Ⅲ～Ⅳ期LC3 mRNA表达显著低于Ⅰ～Ⅱ期（ P＜0.05）。在月经周期不同时相，正常内膜组增殖期的LC3 mRNA表达明显高于分泌期（ P＜0.05） ，卵巢子宫内膜异位症在位内膜组、异位内膜组增殖期与分泌期比较，差异无统计学意义（ P＞0.05）。结论自噬参与了卵巢子宫内膜异位症的发病，自噬活性的改变可能与子宫内膜异位症的发展及病损程度相关。

Objective To study the expression of autophagy gene LC 3 in the ovarian endome-triosis so as to explore the correlation and significance between LC 3 and the occurrence and de-velopment of the ovarian endometriosis .Methods LC3 mRNA quantitative analysis was car-ried out for the normal endometrial tissue , the eutopic endometrial tissue and ectopic endome-trial tissue of ovarian endometriosis patients by Real-time PCR, its relation with clinical stages was investigated .Results The expression of LC 3 mRNA had a significant difference （ P0.05）.The expression of LC3 mRNA in stage Ⅲ~Ⅳ was significantly lower than that of stageⅠ~Ⅱin the ectopic endometrium group （ P0.05 ） .Conclusion Autophagy partici-pates in the attack of ovarian endometriosis .The changes of autophagy activities may be related to the degree of development and impairment of endometriosis .