果蔗SoSGT1与Gibberella fujikuroi侵染下果蔗叶片蛋白的互作研究

Studies on Interaction between SoSGT1 and Proteins in Leaves of Chewing Cane Infected by Gibberella fujikuroi

为了解果蔗(Saccharum officenarum L.)在防御Gibberella fujikuroi过程中与So SGT1互作的蛋白,利用GST蛋白标签载体p GEX-6p-1与果蔗So Sgt1基因构建p GEX-6p-1-sgt1表达载体,诱导表达GST-So SGT1融合蛋白,并通过GST pull down技术捕捉到7个与So SGT1互作蛋白。这些蛋白的功能主要归类为信号转导、抗逆与能量代谢相关蛋白,其中一些蛋白可能与果蔗So SGT1直接互作,如HSP90与RAR1蛋白,另外一些蛋白与果蔗So SGT1可能产生次级互作。在G.fujikuroi侵染果蔗‘福农’叶片时,HSP70、14-3-3蛋白、2-半胱氨酸-过氧化物酶与吡哆醇生物合成蛋白的编码基因呈上调表达,初步说明这些蛋白可能与So SGT1蛋白互作共同参与了果蔗防御梢腐病病原G.fujikuroi的病理过程。

In order to understand the interaction proteins with SoSGT1 during chewing cane （Saccharum officenarum L.） in denfenseGibberella fujikuroi,SoSgt1and vector pGEX-6p-1 was used to construct vector pGEX-6p-1-sgt1, which expressed fusion protein of GST-SoSGT1. Seven proteins were obtained by GST pull down. The function of these proteins was classiifed into signal transduction, stress resistance and energy metabolism, in which might directly interact with SoSGT1, such as HSP90 and RAR1, and others might indirectly interact with SoSGT1. When the leaves of chewing cane ‘Fuan’ were infected byG. fujikuroi, the expression of coding genes of HSP70, 14-3-3 protein, 2-Cys-peroxiredoxin and pyridoxine biosynthesis protein was up-regulated, which indicated that these proteins might interact with SoSGT1 and associate with the resistance of chewing cane toG. fujikuroi.