葡萄胎病理相关新基因F10与绒癌细胞系JEG-3成瘤性的关系

Role of hydatidiform mole-related gene F10 in the tumorigenicity of choriocarcinoma cell line JEG-3

目的：探讨葡萄胎病理相关新基因F10与绒癌细胞系JEG-3裸鼠皮下成瘤的关系。方法通过细胞转染技术及RNA干扰技术，分别建立和筛选出F10基因稳定过表达及沉默的绒癌细胞系JEG-3。取SPF级裸鼠（4~5周龄）30只，随机均分为JEG-3 F10过表达组、JEG-3 F10沉默组、JEG-3未处理组（n=10），分别接种F10基因过表达的JEG-3细胞、F10基因沉默的JEG-3细胞和未处理的JEG-3细胞株5×107个于颈背部皮下。接种后每3~4 d称量裸鼠质量观察，记录肿瘤发生时间，观察皮下肿瘤的生长情况。绘制在体肿瘤生长曲线，计算各组裸鼠的成瘤率。结果3组的成瘤率均为100%（10/10）。JEG-3 F10过表达组、JEG-3 F10沉默组、JEG-3未处理组的成瘤时间分别为6.2±0.78、7±2.49、6.3±0.67 d，组间比较无统计学差异（F=0.781，P=0.468）。JEG-3 F10过表达组肿瘤的在体生长速度较JEG-3 F10沉默组、JEG-3未处理组增快，差异有统计学意义（P〈0.05），JEG-3 F10未处理组肿瘤的在体生长速度较JEG-3 F10沉默组快（P〈0.05）。细胞接种5周后处死小鼠，取瘤组织称重，JEG-3 F10过表达组、JEG-3 F10沉默组、JEG-3未处理组肿瘤重量分别为571.1±221.10 mg、136.2±66.25 mg、354.5±116.23 mg，组间比较存在统计学差异（F=21.199，P=0.000）。结论 F10基因与绒癌细胞系JEG-3的增殖调节有关，可增强JEG-3细胞系在裸鼠体内的致瘤性。

Objective To explore the role of the hydatidiform mole-related gene F10 in the tumorigenicity of choriocarcinoma cell lines JEG-3 in nude mice. Methods Choriocarcinoma JEG-3 cell lines with stable F10 gene over-expression and F10 gene silencing were established using cell transfection and RNA interference techniques, respectively. Thirty SPF nude mice （4-5 weeks old） were equally randomized into F10 over-expression group, control group, and F10 gene-silenced group for subcutaneous injection of 0.2 ml cell suspension （5 × 107 cells） of F10 gene over-expressing JEG-3 cells, non-treated JEG-3 cells, and F10 gene-silenced JEG-3 cells, respectively. The mice were observed and weighed every 3-4 days, and the tumor formation time was recorded to draw the tumor growth curve and calculate the tumor formation rate. Results The tumor formation rates were 100% in all the 3 groups. No significant difference was found in the tumor formation time among the F10 over-expression, F10-silenced and control groups （6.2 ± 0.78 vs 7 ± 2.49 vs 6.3 ± 0.67 days; F=0.781, P=0.468）. A significantly greater tumor growth rate was noted in the F10 over-expression group compared with the other two groups （P〈0.05）, and the growth rate was significantly slower in F10-silenced group than in the control group （P〈0.05）. The subcutaneous tumor weight at 5 weeks after JEG-3 cell injection differed significantly among F10 over-expression, F10-silenced and control groups （571.1 ± 221.10 vs 136.2 ± 66.25 vs 354.5 ± 116.23 mg; F=21.199, P=0.000）. Conclusion F10 gene plays a role in the regulation of choriocarcinoma JEG-3 cell proliferation and might enhance its tumorigenicity in nude mice.