不同年龄女性人群血液中的Dkk-1水平与骨密度相关性的分析研究

Relevance analysis of serum Dkk-1 expression and bone mineral density in different ages of female populations

目的：探讨不同年龄女性人群血液中的Dickkopf-1（ Dkk-1）水平与骨密度之间的相关性。方法：选取2013年3月～2014年6月在我院行体检的100例年龄处于20～80岁之间的女性志愿者，按照不同年龄分为青年组（20～39岁）、中年组（40～59岁）和老年组（60～80岁），从中年女性组中抽取45～55岁25人，分为绝经期女性组和未绝经女性组。采用酶联免疫吸附法测3组志愿者血清Dkk-1的表达水平，采用双能X线骨密度测量仪测定3组志愿者全身矿含量，分析不同年龄女性以及绝经与否女性血清Dkk-1水平的差异及Dkk-1水平与骨密度的关系。结果：随着年龄的增长，人群血清Dkk-1水平相应增高（P＜0.05），骨密度相应降低（P＜0.05）；3组志愿者中，血清Dkk-1水平与骨密度均呈负相关（P＜0.05）。45～55岁年龄范围内，绝经组女性血清Dkk-1与未绝经妇女相比，Dkk-1水平与骨密度降低的相关性更为密切（ P＜0.05）。结论：人体随着年龄的增加，血清Dkk-1水平会上升，并出现骨密度下降，有发生骨质疏松症的风险。相同年龄范围妇女中，绝经后妇女Dkk-1水平表达较高，骨密度降低更明显，有更大的发生骨质疏松症危险。 Dkk-1表达的增加同时可抑制骨形成和促进骨吸收，有望成为预防和治疗骨质疏松症治疗的一个新靶点。

AIM：To conduct the relevance analysis of serum Dickkopf-1 （Dkk-1） and bone mineral density （BMD） in the different ages of female populations.METHODS： The women volunteers （n=100, 20~80 years old） were selected and divided into young group （20~39 years old）, middle age group （40~59 years old） and elderly group （60~80 years old）.The serum levels of Dkk-1 in the 3 groups of volunteers were detected by ELISA.In the middle age group, 25 people of 45~55 years old were selected and divided into postmenopausal group and premenopausal group to de-tect the serum level of Dkk-1 in the 2 groups of volunteers by ELISA.The BMD was measured by the method of dual energy X-ray absorptiometry.The differences of Dkk-1 expression levels among different ages of female populations, and the rele-vance with BMD were compared.RESULTS：With the increase in age, the serum Dkk-1 expression level increased （ P〈0.05）, and BMD were reduced （P〈0.05）.The blood level of Dkk-1 and BMD negatively correlated （P〈0.05） in the 3 groups of volunteers.The serum levels of Dkk-1 and BMD had stronger negative correlation in postmenopausal women group than that in premenopausal women group.CONCLUSION：With the increase in age, the expression level of serum Dkk-1 increases and the BMD level decreases, which contribute to a risk of osteoporosis.In the same age range, the postmeno-pausal women express higher level of Dkk-1, and the decreased BMD is more obvious, which contribute to a greater risk of osteoporosis.The increased level of Dkk-1 also inhibits bone formation and promotes bone resorption.It may become a new target for preventing and treating osteoporosis.