摘要
目的:探讨不同年龄女性人群血液中的Dickkopf-1( Dkk-1)水平与骨密度之间的相关性。方法:选取2013年3月~2014年6月在我院行体检的100例年龄处于20~80岁之间的女性志愿者,按照不同年龄分为青年组(20~39岁)、中年组(40~59岁)和老年组(60~80岁),从中年女性组中抽取45~55岁25人,分为绝经期女性组和未绝经女性组。采用酶联免疫吸附法测3组志愿者血清Dkk-1的表达水平,采用双能X线骨密度测量仪测定3组志愿者全身矿含量,分析不同年龄女性以及绝经与否女性血清Dkk-1水平的差异及Dkk-1水平与骨密度的关系。结果:随着年龄的增长,人群血清Dkk-1水平相应增高(P<0.05),骨密度相应降低(P<0.05);3组志愿者中,血清Dkk-1水平与骨密度均呈负相关(P<0.05)。45~55岁年龄范围内,绝经组女性血清Dkk-1与未绝经妇女相比,Dkk-1水平与骨密度降低的相关性更为密切( P<0.05)。结论:人体随着年龄的增加,血清Dkk-1水平会上升,并出现骨密度下降,有发生骨质疏松症的风险。相同年龄范围妇女中,绝经后妇女Dkk-1水平表达较高,骨密度降低更明显,有更大的发生骨质疏松症危险。 Dkk-1表达的增加同时可抑制骨形成和促进骨吸收,有望成为预防和治疗骨质疏松症治疗的一个新靶点。
AIM:To conduct the relevance analysis of serum Dickkopf-1 (Dkk-1) and bone mineral density (BMD) in the different ages of female populations.METHODS: The women volunteers (n=100, 20~80 years old) were selected and divided into young group (20~39 years old), middle age group (40~59 years old) and elderly group (60~80 years old).The serum levels of Dkk-1 in the 3 groups of volunteers were detected by ELISA.In the middle age group, 25 people of 45~55 years old were selected and divided into postmenopausal group and premenopausal group to de-tect the serum level of Dkk-1 in the 2 groups of volunteers by ELISA.The BMD was measured by the method of dual energy X-ray absorptiometry.The differences of Dkk-1 expression levels among different ages of female populations, and the rele-vance with BMD were compared.RESULTS:With the increase in age, the serum Dkk-1 expression level increased ( P〈0.05), and BMD were reduced (P〈0.05).The blood level of Dkk-1 and BMD negatively correlated (P〈0.05) in the 3 groups of volunteers.The serum levels of Dkk-1 and BMD had stronger negative correlation in postmenopausal women group than that in premenopausal women group.CONCLUSION:With the increase in age, the expression level of serum Dkk-1 increases and the BMD level decreases, which contribute to a risk of osteoporosis.In the same age range, the postmeno-pausal women express higher level of Dkk-1, and the decreased BMD is more obvious, which contribute to a greater risk of osteoporosis.The increased level of Dkk-1 also inhibits bone formation and promotes bone resorption.It may become a new target for preventing and treating osteoporosis.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2015年第5期906-910,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81472089)