选择性敲除小鼠神经细胞Adam10基因导致大脑皮质发育不良

Conditional Knockout Adam10 Gene of Neuronal Cell in Mouse Leads to Cortical Dysplasia

目的:研究Adam10基因在小鼠神经系统发育中的作用。方法:利用Cre-loxp转基因鼠技术,将Gfapcre转基因鼠和Adam10lox/lox转基因鼠杂交,得到神经细胞特异性Adam10基因敲除鼠(Gfapcre-Adam10lox/lox),在出生后第14天对大脑切片行HE染色,观察Adam10基因敲除后神经系统发育情况。结果:选择性敲除小鼠神经细胞Adam10基因(Gfapcre-Adam10lox/lox),小鼠可存活至出生后3周左右,部份小鼠大脑皮质单侧或双侧缺失,未出现皮质缺失的小鼠大脑皮质经HE染色提示出现层次结构紊乱。结论:Adam10基因在小鼠神经系统发育中具有重要作用,选择性敲除神经细胞Adam10基因后导致小鼠大脑皮质缺失或层次结构紊乱。

Objective： To investigate the role of Adam10 gene in mouse central nervous system（CNS） development. Methods： Gfapcremice were mated to Adam10lox/loxmice using cre-loxp conditional gene knockout technique,and mice of conditional knockout Adam10 gene of neuronal cells（Gfapcre-Adam10lox/lox） were produced. At postnatal day of fourteen, mouse brain sections were prepared and HE staining was used to observe CNS development in Adam10 gene knockout mice. Results： By conditional knockout Adam10 gene of neuronal cell in mice（Gfapcre-Adam10lox/lox）, mice could survive to about three weeks after birth. A tiny fraction of mice showed cortical defect,and developed cerebral cortex showed disorders of cortical lamination by HE staining. Conclusion： Adam10 gene plays an important role in CNS development. Conditional knockout Adam10 gene of neuronal cell in mouse leads to cortical defect or dysplasia.