摘要
对实验室保藏的一株维生素K2(MK-7)高产菌的摇瓶发酵条件进行优化,结果表明菌体在静置培养状态下比在摇瓶培养状态下能够合成更多的维生素K2;同时发现玉米粉经过高温淀粉酶液化之后非常适合作为合成维生素K2的碳源。最后利用响应面法对发酵培养基中主要因素的最适宜水平及其交互作用进行了研究与探讨,优化后维生素K2产量由7.09 mg·L-1提高到了67.22mg·L-1,高于文献报道值。
The fermentation condition optimization procedure of a high vitamin K2（ MK-7） producing strain preserved in the laboratory was conducted for higher vitamin K2 production. The results showed that this strain could synthesize more vitamin K2 in static culture condition than that in shaking culture. And when the corn meal was liquefied by thermostable amylase,the liquified corn meal was very suitable for the synthesis of vitamin K2. At last,the response surface methodology was applied to find the most appropriate level of major factors. After optimization the concentration of vitamin K2 reached as high as 67. 22 mg·L^- 1,much higher than that without optimization of 7. 09 mg·L^- 1and the reported.
出处
《工业微生物》
CAS
CSCD
2015年第3期14-23,共10页
Industrial Microbiology
关键词
维生素K2
人发酵条件优化
玉米粉水解液
响应面法
vitamin K2
fermentation condition optimization
liquified corn meal
response surface methodology
