四神丸和葛根芩连片对结肠炎小鼠炎性细胞因子的影响

Effects of Sishen Pill and Gegen Qinlian Tablet on Inflammatory Cytokines in Mice with Colitis

目的探讨四神丸和葛根芩连片对硫酸葡聚糖钠(DSS)诱导的小鼠急性和慢性结肠炎细胞因子的影响。方法小鼠自由饮用4%DSS连续5 d,制备急性结肠炎模型;小鼠4次循环饮用3%DSS制备慢性结肠炎模型。急性或慢性结肠炎实验小鼠均随机分为对照组、DSS急性或慢性模型组(模型组)、四神丸组、葛根芩连片组。急性模型小鼠于DSS饮水次日开始给药,连续8 d;慢性模型于DSS饮水2次循环后开始给药,连续16 d。ELISA检测各组小鼠结肠培养上清液中γ-干扰素(IFN-γ)、IL(白细胞介素)-17A及IL-22的含量。结果急性结肠炎模型小鼠结肠培养上清液中IFN-γ、IL-17A及IL-22含量均明显增加(P<0.01),葛根芩连片能显著降低急性结肠模型小鼠IFN-γ、IL-17A的含量(P<0.05),四神丸则能显著降低IL-17A的含量(P<0.05)。慢性结肠炎模型小鼠结肠培养上清液中IFN-γ、IL-17A较对照组显著升高(P<0.01);葛根芩连片及四神丸有降低二者的趋势。与对照组比较,四神丸可升高IL-22含量(P<0.05)。结论葛根芩连片和四神丸通过降低急性结肠炎模型小鼠IFN-γ、IL-17A含量达到其治疗作用,四神丸通过促进IL-22升高发挥其治疗慢性结肠炎的作用。

Objective To explore the effects of Sishen Pill and Gegen Qinlian Tablet on cytokines of acute and chronic colitis induced by dextran sulfate sodium （DSS） in mice. Methods Mice freely drank 4%DSS dissolved in drinking water for continuous 5 days to establish acute colitis model. Mice circularly drank 3% DSS for 4 times for the establishment of chronic colitis model. In the acute or chronic colitis experiment, mice were randomly divided into control group, acute and chronic model groups, Sishen Pill group, and Gegen Qinlian Tablet group. Acute model group was administrated one day after DSS drinking for 8 days. Chronic model group was administrated after the second time of circular drinking for 16 days. ELISA was used to detect the contents of IFN-γ, IL-17 and IL-22 in cultural supernatant of mouse colon. Results Contents of IFN-γ, IL-17A and IL-22 in cultural supernatant increased significantly in acute models （P〈0.01）. Gegen Qinlian Tablet can significantly decrease the contents of IFN-γ and IL-17A of acute models （P〈0.05）. Sishen Pill can significantly decrease the content of IL-17A （P〈0.05）. IFN-γand IL-17A in cultural supernatant in chronic model mice significantly increased compared with control group （P〈0.01）. Sishen Pill and Gegen Qinlian Tablet inhibited the contents of IFN-γ and IL-17A. Compared with control group, Sishen Pill significantly increased the content of IL-22 （P〈0.05）. Conclusion Sishen Pill and Gegen Qinlian Tablet can treat colitis by decreasing the contents of IFN-γ and IL-17A in acute colitis model mice;Sishen Pill can treat chronic colitis by promoting IL-22 to increase.