AtHsfAla对拟南芥幼苗在干旱中生理指标的影响

Effect of Heat Shock Factor AtHsfAla on Physiological Indexes in Arabidopsis Thaliana Seedlings in Drought

以过量表达AtHsfAla及AtHsfAla基因沉默的转基因植株为材料,通过控制土壤水分进行干旱处理,结果显示,过量表达型干旱处理后的存活率最大,而基因沉默型最小,表明AtHsfA1a能增强植物的抗旱性.为了从生理水平研究AtHsfA1a提高抗旱性的机制,研究发现过量表达型的叶片相对含水量和叶绿素含量最高,而沉默型最低.不同基因型植株的H2O2和MDA的含量正好相反,说明AtHsfA1a通过保护细胞膜而减小细胞所受氧化伤害来提高抗旱能力.

In this study,transgenic Arabidopsis thaliana plants with over expressing AtHsfA1a and silencing mutant of Arabidopsis AtHsfA1a were used as experimental material to be handled in drought by controlling the soil water.The results showed that the survival percents of the plants with over expressing AtHsfA1a were the highest,but the silencing mutant was the lowest,indicating that AtHsfA1a could enhance the drought resistance of Arabidopsis plants.In order to study the mechanism of drought resistance improved by heat shock factor AtHsfAla in physiological level,it was found that water content and chlorophyll content of the plants with over expressing AtHsfA1a were highest,while the silencing mutant were lowest.The contents of H2 O2 and MDA in different genotypes were just the opposite,showing that AtHsfA1a could reduce oxidative stress thus protect cell membranes from damage and enhance antioxidant capacity.