强直性脊柱炎患者外周记忆性T细胞亚群及功能初探

Preliminary exploration of peripheral memory T cell subsets and their functions in ankylosing spondylitis

通过分析强直性脊柱炎患者外周血CD4+和CD8+记忆性T细胞亚群比例和产生细胞因子IFN-γ的能力,探讨记忆性T细胞在强直性脊柱炎发病中的作用。流式细胞术检测强直性脊柱炎患者外周血CD4+和CD8+记忆性T细胞亚群分布和分泌IFN-γ的记忆性T细胞百分比。强直性脊柱炎患者外周CD4+和CD8+记忆性T细胞的比例和正常对照组相比,无差异,进一步比较效应型(TEM)和中央型记忆性T细胞(TCM)在CD4+和CD8+亚群中的分布,发现CD4+TEM及CD4+TCM与正常人相比无明显差异(P>0.05),而CD8+TCM与正常人相比显著升高(P<0.05),CD8+TEM与正常对照相比则明显下降(P<0.05)。同时,强直性脊柱炎患者外周CD4+和CD8+记忆性T细胞分泌细胞因子IFN-γ的细胞比例与正常人相比显著下降(P<0.05)。强直性脊柱炎患者在疾病活动期状态下,其外周CD8+记忆性T细胞的亚群分布发生异常,同时CD4+TM和CD8+TM的数量虽然没有变化,但是其产生细胞因子IFN-γ的功能下降。这可能与患者对感染的易感性提高有一定关联。

To explore the role of peripheral memory T cells in the pathogenesis of ankylosiing spondilitis（AS）,we investigated the capability of producing IFN-γof memory CD4^＋ and CD8^＋T cells and the ratio of CD4^＋and CD8^＋memory T cells.We found that the frequencies of CD4^＋and CD8^＋TMin AS were comparable to those in normal controls（P〉0.05）.We further detected the distribution of TEMand TCMin CD4^＋and CD8^＋T cells,and found that the frequencies of TEMand TCMin the peripheral CD4^＋T cells were similar to those in the normal controls（P〉0.05）.However,the frequency of CD8^＋TCMwas significantly higher in AS Patients than that in the normal controls while the frequency of CD8^＋TEMwas lower than that in the normal controls（P〈0.05）.The capability to producing IFN-γby CD4^＋and CD8^＋TMin AS patients were significantly impaired when compared to normal controls（P〈0.05）.In conclusion,during active period of AS there exists the altered CD8^＋memory T-cell homeostasis in periphery.Although there is no change in the quantity of CD4^＋TM and CD8^＋TMsubsets,their functionality has decreased.This is probably dedicated to the susceptibility to infection in AS patients.The mechanism remains to be investigated further.