固有免疫信号分子hsa-miR-181a调节脑卒中作用的生物信息学分析

Bioinformatical analysis of the role of innate immune signaling molecule hsa-miR-181ain regulation of signal pathways in stroke

对固有免疫信号分子hsa-miR-181a进行生物信息学分析,以期探讨其调节脑卒中的生物学途径和信号通路,为hsamiR-181a的实验验证及功能研究提供理论指导。利用TargetScan 6.0、miRWalk、miRanda、PicTar、Genecards数据库预测所得的hsa-miR-181a与脑卒中所相关的靶基因,通过BINGO及DAVID对脑卒中相关靶基因集合进行基因本体(gene ontology,GO)和信号通路分析。hsa-miR-181a可与固有免疫基因TLR4相互作用,并同时作用于与脑卒中相关的其它靶基因,通过调节细胞途径,影响代谢过程等生物学途径,神经营养因子信号转导等KEGG信号通路发挥作用。固有免疫信号分子hsa-miR-181a可通过调节细胞途径,影响代谢过程等生物学途径,神经营养因子信号转导等信号通路调节脑卒中,为进一步实验和功能验证提供生物信息学指导。

We set out this study to analyst signaling molecule hsa-miR-181a of the innate immune system to explore its role in the regulation of biological pathway and signal transduction in stroke and provide bioinformatical analysis for experimental verification and functional study. We first predict the target genes related to hsa-miR-181a in the patient with stroke via TargetScan 6, miRWalk, miRanda, PicTar, Genecards database. The stoke-related target genes were then analyzed by gene ontology and signaling pathway based on BINGO and DAVID. The results showed that hsa-miR-181a could interact with the innate immunity gene TLR4 and the other stroke-related genes. They could exert their functions through regulating cellular pathways and affecting metabolic processes, and further display their neurotrophin signaling transduction in the KEGG signaling pathway. We conclude that the innate immune signaling molecule hsa-miR-181a might regulate stroke via cellular pathways, metabolism process and neurotrophie signaling transduction pathways. The method might provide biological information for further experimental and functional verification.