IL-1β对人体皮肤成纤维细胞增殖的影响

Effects of Interleukin-1β on Proliferation of Fibroblast in Vitro

目的探讨白介素-1β(interleukin-1β,IL-1β)对人体皮肤成纤维细胞增殖的影响及其可能机制。方法胶原酶消化法提取人皮肤成纤维细胞(human skin fibroblasts,HFB)。分别用0,0.04,0.2,1与5 ng/m L浓度的IL-1β培养HFB 24 h,以0 ng/m L浓度的IL-1β培养HFB 24 h为对照组,采用流式细胞分析法测定各组细胞周期分布情况,MTT法测定各组细胞增殖情况。结果细胞周期分析结果显示,各实验组与对照组相比,S期所占比例增加,且随着培养液中IL-1β浓度增高,S期所占比例上升。MTT法测定结果表明,各实验组OD值均比对照组高,且实验组OD值随着培养液中IL-1β浓度增高而增高。细胞周期和MTT结果均表明,IL-1β浓度为5 ng/m L时,其促进细胞增殖的效果最明显。结论 IL-1β浓度是影响HFB增殖的重要因素,本实验为创伤后表皮细胞和真皮细胞大量分泌IL-1β并促进HFB增殖的现象提供了参考。

Objective To study the effects of interleukin-1β on proliferation of human fibroblasts （HFB） in vitro, and the possible mechanisms. Methods HFB were isolated using collagenase digestion method, and cuhured with IL-1β at concentrations of 0.04, 0. 2, 1 and 5ng/mL for 24 hours. The HFB cultured without IL-1β served as controls. Flow cytometry was used to determine cell cycle, and MTT assay was used to assess the proliferation of HFB. Results In comparison with the controls, IL-1β increased both the S-phase fraction of HFB and OD values, as determined by flow cytometry and MTT assay, respectively. Both the portion of S- phase HFB and OD values were positively correlated the IL-1β concentrations. Both flow cytometry and MTT results demonstrated that IL-1β at the concentration 5 ng/mL was most effective in stimulating HFB prolifera- tion. Conclusion The concentration of IL-1β is a key factor affecting HFB proliferation. The present studies explain why that both epidermal and dermal cells secrete IL-1β and stimulate HFB proliferation following injury.