水稻二半乳糖甘油酯合成酶DGD响应热胁迫的生物信息学和基因表达分析

Bioinformatic analysis and gene expression of digalactosyl-diacylglycerol synthase(DGD) on there sponse of thermal stresson rice

利用比较基因组学方法获得5个水稻二半乳糖甘油酯合成酶（Os DGD）同源基因;运用生物芯片分析,发现Os DGD参与对热胁迫和干旱胁迫的应答;采用实时定量PCR技术,分析5个Os DGD在热胁迫幼穗6~7期日本晴、9311和N22水稻剑叶中的表达特征,发现Os DGD1、Os DGD2和Os DGD3在耐热品种N22中的表达明显受到热胁迫诱导,由此推测,Os DGD可能参与了水稻的耐热信号传导。

Five homologous genes of digalactosyl-diacylglycerol synthase（Os DGD） were obtained through the approach of comparative genomics from rice. From the analysis of biochip expression, the result indicated that Os DGD genes were involved in thermal and drought stress. By using real-time quantitative PCR analysis and adopting flag leaves of the young panicle at 6–7 stages of cultivar Oryza sativa Nipponbare 9311 and N22 as materials, the expression levels of 5 Os DGD genes in response to thermal stress showed that Os DGD1, Os DGD2 and Os DGD3 were obviously induced by thermal stress in N22 line, which had higher basal thermo-tolerance level. By taken all test results into consideration, we inferred that Os DGD genes were involved in thermal stress of signal transduction pathway in rice.