抗锰细菌的分离鉴定及其对生猪养殖粪水中锰的去除效果

Isolation,identification of a manganous salt resistant bacterium and manganese removal from pig-breeding fecal wastewater

为筛选对锰污染环境进行微生物治理的抗锰菌,从湘潭锰矿的矿石和锰尾矿坝采集土样,通过含Mn SO4的固体平板法分离、纯化抗锰细菌,依据形态学特性和16S rDNA序列的系统发育分析对抗锰菌株进行鉴定;抗锰菌株在含0.005、0.010、0.015 mol/L Mn SO4的培养液中生长后,用火焰原子吸收光谱法测定抗锰菌培养液的离心上清液中Mn2+的含量,计算Mn2+的去除率;通过正交试验,以生猪养殖粪污废水原液直接接种抗锰细菌液体菌种,检测抗锰菌的除锰能力。结果表明:1)筛选、分离出1株抗锰菌,将其定名为KM01,经鉴定,该菌为一种沙雷氏菌;2)在含0.005、0.010、0.015 mol/L MnSO4的培养液中,菌株KM01能吸附Mn2+,并以黑褐色沉淀形式沉降析出,28℃下培养液中Mn2+的沉降去除率分别为94.8%、95.8%和89.1%;3)在28℃、p H 7与接种量1%时,生猪养殖废水中Mn2+的沉降去除率分别为94.8%、95.8%和89.1%;3)在28℃、p H 7与接种量1%时,生猪养殖废水中Mn2+的吸附沉降去除率为80.5%,表明抗锰沙雷氏菌KM01在生猪养殖粪污废水的沉降除锰中具有应用潜力。

To screen manganous-salt resistant bacteria for the microbial treatment of manganese polluted environment. The soil samples were collected from the manganese ore yard and manganese tailing dams in the Xiangtan Manganese Industry Co. Lid.. The manganous salt-resistant bacteria were isolated on manganous sulfate containing solid plate. The morphology characteristics and phylogenic analysis of the 16 S r DNA sequence were used for the identification of the strain KM01. The flame atomic absorption method for the determination of the manganese concentration in centrifuged broths containing 0.005 mol/L, 0.01 mol/L and 0.015 mol/L of manganous sulfate was used to calculate the removal rate of manganese. Through an orthogonal experimental design, the liquid inocula of KM01 were directly inoculated into the crude pig-breeding fecal wastewater was used to detect the manganese removal capacity of the strain. 1） A manganous salt-resistant bacterium KM01 was isolated and identified as Serratia sp. 2） In the broths containing manganous sulfate, KM01 could biosorb manganous ion and produce dark brown precipitate. The removal rate of manganese reaches 94.8%, 95.8% and 89.1%, respectively. 3） The removal rate of manganese in the wastewater reaches 80.5% under the condition of 28 ℃, p H 7 and 1% of inoculum size. The manganous salt-resistant strain Serratia sp. KM01 has potential application in the precipitation and removal of manganese in organic wastewater containing manganous ion the same as pig-breeding fecal wastewater.