酸碱条件对枯草芽胞杆菌FJAT-14254代谢物产生的影响

Effects of Initial p H Value for the Fermentation of Bacillus subtilis FJAT-14254 on the Generation of Metabolites Substances

为考察酸碱条件对枯草芽胞杆菌FJAT-14254代谢物产生的影响,采用气相色谱-质谱联用分析枯草芽胞杆菌FJAT-14254菌株的胞内成分。结果表明:通过谱库扫描得到32种高匹配率代谢物的初步鉴定结果,主要包括氨基酸类、酸类、烷烃类等,其中含量相对较高的有7种;枯草芽胞杆菌FJAT-14254代谢物的种类和含量与其培养环境的酸碱度变化呈一定的相关性,强酸性(p H=3)条件下培养的胞内代谢物具有特异性,而接近中性(p H=5、7)条件及碱性(p H=9、11)条件下培养胞内代谢物则相似。由此推断,不同p H条件影响发酵终点的p H,进而影响枯草芽胞杆菌FJAT-14254代谢物的产生。

To study the relationship between pH of culture medium and intracellular metabolic of Bac illus subtilis FJAT-14254. Intracellular metabolites of B. subtilis FJAT-14254 were analyzed by GC-MS. 564 metabolites were detected. 31 metabolic markers including hexadecanoic acid, was obtained by data mining. The biochemical substances of B. subtilis FJAT-14254, produce in different pH conditions, had high specificity. The change of metabolites in different pH conditions was complex, not only linear relation. This research provided preliminary study of stress resistance of B. subtilis in metabolic level.