检材采集与保存方式对DNA提取效率的影响

Effect of Sample Collection and Preservation on DNA Yield

目的研究检材采集与保存方式对DNA提取效率的影响。方法使用生物检材采集与保存套管棉签、尖头棉签和普通医用棉签采集血样,分别放置于生物检材采集与保存套管或纸质物证袋中保存1周。剪取全部血样于96孔板中,用磁珠法结合自动化工作站提取DNA,以ABI7500型荧光定量PCR仪定量。结果生物检材采集与保存套管采集保存的血样所提取的DNA浓度平均为(2.54±0.63)ng/μL,而尖头棉签、普通医用棉签采集并分别用纸袋保存的血样提取的DNA浓度平均为(2.06±0.44)ng/μL和(0.93±0.59)ng/μL。结论生物检材采集与保存套管较之于尖头棉签或普通医用棉签采集、纸袋保存方式,其获得的DNA浓度显著提高,DNA提取率高。

ObjectiveTo explore the effect of sample collection and preservation on DNA yield.MethodsThe fresh anti-freezing blood from 30 volunteers was respectively dropped onto a swab from the casing for collection and preservation of biological sample, and the ones of both the cuspidal and the common medical. Each of the blood specimens was separately preserved in the casing or paper bags for a week. Every piece of DNA from the blood specimens was extracted by magnetic beads method in combination of automation workstation and was quantiifed by ABI 7500 Real Time System.ResultsThe average DNA concentration was （2.54±0.63） ng/μL for the blood specimens collected and preserved with the casings, （2.06±0.44） ng/μL and （0.93±0.59） ng/μL for those blood specimens which were collected with cuspidal swabs and common medical ones and followed to preserve in paper bags. The DNA yield was obviously higher using the casings than that of either the cuspidal swabs or the common medical ones to collect and then preserve in paper bags.ConclusionsThis test suggests that it is better to choose the casings for collection and preservation of biological sample than the cuspidal and the common medical swab.