正交设计-重复试验优选桔皮精油的酶解提取工艺

Optimization of Enzymolysis Extraction Technology of Orange Peel Essential Oil by Orthogonal Design-Repeated Test

目的优选酶解-水蒸气蒸馏法提取桔皮中桔皮精油的工艺。方法采用酶解-水蒸气蒸馏提取法,以桔皮精油的得率为考察指标,通过正交设计-重复试验优化复合酶配比、pH、酶解温度、蒸馏时间4个因素对精油得率的影响来确定最佳提取工艺参数。结果酶解-水蒸气蒸馏法提取桔皮中精油的最佳条件为果胶酶与纤维素酶配比为1∶2,pH=4,酶解温度为50℃,蒸馏时间为3h;其中pH对精油得率的影响最大,其次为蒸馏时间,酶解温度影响较小,而复合酶的配比影响程度最低。干桔皮中精油的得率为2.18%。结论采用酶解-水蒸气蒸馏提取法能提高桔皮精油得率。

Objective To optimize the enzymolysis- steam distillation extraction technology of essential oil from orange peels. Methods The enzymolysis- steam distillation extraction method was adopted with the orange peel essential oil yield as the investigation index. The influence of 4 factors of compound enzymatic ratio, PH, enzymolysis temperature and distillation time on the essential oil yield were optimized by the orthogonal design- repeated test for determining the optimal technological parameters. Results The optimal extraction conditions by the enzymolysis- steam distillation method were as follows： the ratio of pectinase to cellulose was 1：2, pH =4, the enzymolysis temperature was 50 ℃ and the distillation time was 3 h. The yield rate of essential oil in the dried orange peels was 2. 18%. pH had greatest influence on the yield rate of essential oil, followed by the distillation time, the enzymolysis temperature had smaller influence, and the influence degree of compound enzymatic ratio was lowest. Conclusion Adopting the enzymolysis- steam distillation extraction method can increase the yield rate of essential oil from orange peels.