1-磷酸鞘氨醇对原代骨髓间充质干细胞增殖和分化的影响

Effect of Sphingosine 1-Phosphate on Proliferation and Differentiation of Primary Bone Marrow Mesenchymal Stem Cells

目的探讨1-磷酸鞘氨醇(S1P)在骨髓间充质干细胞增殖及诱导分化为血管内皮细胞中的作用。方法采用S1P预处理原代骨髓间充质干细胞(PR-MSCs),并评估S1P对PR-MSCs增殖和分化的影响。结果 S1P能显著促进PR-MSCs的增殖,促进PR-MSCs经血管内皮生长因子(VEGF)诱导分化为血管内皮细胞,这与经S1P处理后的PR-MSCs表达VEGF及Angiopointin-1的m RNA增多有关,同时P-ERK1/2高表达于S1P预处理后的PR-MSCs中。结论 S1P能促进PR-MSCs的增殖和分化为血管内皮细胞,并且S1P通过ERK通路影响PR-MSCs的旁分泌,这将促进PR-MSCs的临床应用以及加强对S1P作用机制的了解。

Objective To investigate the effects of sphingosine- 1 -phosphate （S1P） on the proliferation and differentiation of bone marrow mesenchymal stem cells（MSCs） into vascular endothelial cells. Methods Primary bone marrow MSCs （PR-MSCs） were pre-treated with S1P and the effects of SIP on the proliferation and differentiation of PR- MSCs was evaluated. Results The proliferation capability of PR- MSCs was significantly enhanced by SIP. Furthermore, SIP accelerated the differentiation of PR- MSCs into endothelial cells under vascular endothelial growth factor （VEGF） induction, which was related with increasing mRNA production of VEGF and Angiopointin-1 in S1P-PR-MSCs. Meanwhile, P-ERK1/2 was highly expressed in SIP treated PR-MSCs. Conclusion SIP can promote the proliferation and differentiation of PR- MSCs into vascular endothelial ceils. In addition, SIP can affect paracrine of PR- MSCs by activating ERK pathway, which can promote the clinical application of PR- MSCS and strengthen the understanding on SIP action mechanism.