抗氧化剂对高氧损伤新生大鼠脑细胞的保护作用研究

The effect of antioxidants on hyperoxic brain injury in neonatal rats

目的探讨丹参提取物764-3和超氧化物歧化酶（SOD）对高氧损伤新生大鼠脑细胞的保护作用。方法将SD新生大鼠持续暴露于氧浓度≥90%的高氧环境中建立高氧脑损伤模型,然后分别予764-3、SOD进行预防（1-7d）和治疗（8-14d）干预后,计算各组大鼠存活率、体质量和脑湿/干重比值;采用TUNEL技术检测大鼠脑细胞凋亡情况并计算凋亡指数（AI）。结果与空气对照组比较,高氧对照组的AI显著上升（P〈0.01）;干预组的AI均显著低于高氧对照组（P〈0.01）,预防组的AI显著高于治疗组（P〈0.01）。结论 764-3和SOD对于高氧脑损伤均有一定的保护作用,且治疗作用优于预防作用。

Objective To investigate the effect of 764-3 and SOD on hyperoxie brain injury in neonatal rats. Methods Hyperoxic brain injury models were established by exposing to ≥90% oxygen in the neonatal period of Spraque-Dawley rats. After prevention （1-7 d） and treatment（8 14 d）with 764-3 and SOD separately,in all groups survival rate of rat, body weight and wet /dry weight ratio were calculated; apoptosis of rat brain cell were tested by TUNEL technology and apoptotic index（AI）were calculated. Results Compared with the control group, AI in the hyperoxia-exposed group was significantly raised（P〈0. 01）; AI in intervention group was significantly lowered than that in hyperoxia-exposed group,in prevention group was significantly higher than that in treatment group（P〈0.01）. Conclusion Both 764-3 and SOD have similarly protective effect on hyperoxia-induced brain injury,therapeutic effect is superior to the preventive effect.