帕瑞昔布钠抑制补体C3表达减轻骨癌痛的实验分析

目的：探讨帕瑞昔布钠抑制补体C3表达减轻骨癌痛的作用。方法健康雌性SD大鼠120只，体重160-200 g，随机分为4组（每组30只）：假手术组（S组）、骨癌痛组（P组）、帕瑞昔布钠治疗组（T组）和C3补体抑制剂（PEG-Cp40）干预组（C组）。P组、T组和C组均采用胫骨髓腔内注射Walker-256乳腺癌细胞的方法建立大鼠胫骨癌痛模型，S组胫骨骨髓腔内注射等量生理盐水。T组和C组分别鞘内注射帕瑞昔布钠和PEG-Cp40蛋白质10mg。分别于造模前1d及术后3、5、7、14d时测定机械痛阈值，痛阈测定结束后处死大鼠，取脊髓组织，采用免疫比浊法测定脊髓后角中C3补体的表达情况；采用RT-PCR法测定C3的mRNA表达情况。结果与S组比较，P组接种后5-14d机械痛阈下降（P〈0.05），C3含量和mRNA水平明显上调（P〈0.05）；与P组比较，T、C组造模后7-14d机械痛阈升高（P〈0.05），C3含量和mRNA水平明显下降（P〈0.05）；T、C组间未见明显差异（P〉0.05）。结论帕瑞昔布钠可能通过抑制C3作用来减轻骨癌痛。

Objective To evaluate the role of Dynastat Reduce bone cancer pain by inhibiting C3 expression. Methods One hundred and twenty famale SD rats weighing 160-200g were randomly divided into four groups：（n=30）：sham operation group （group S）,BCP group,Dynastat therapy group （group T）and C3 inhibitors group （group C） Tibia bone cancer pain was induced by intra-tibial inoculation of Walker-256 breast cancer cells in groups P,T and C ,group S was injected with normal saline. Dynastat and C3 inhibitors were injected intrathecally in groups N and C respectively. Mechanical pain threshold Was measured at 1 day before and at 3,5,7 and 14 days after BCP.Using immunoturbidimetry and PCR technique to measure C3 content and expression level of mRNA respectively. Results Compared with groups S,mechanical pain threshold was significantly decreased from 5-14 d after inoculation in group p（P〈0.05）,the content of C3 and mRNA level significantly was raised （P〈0.05）;Compared with groups P, mechanical pain threshold was significantly decreased from 5-14 d after inoculation in group T and C. The content of C3 and mRNA level were significantly declined（P〈0.05）.There was no significant statistical difference between group T and C. Conclusion Dynastat may inhibit the bone cancer pain through its inhibiting effect of C3.