鸟氨酸-α-酮戊二酸对腹腔重症感染大鼠肠道免疫功能的影响

Effects of L-Ornithine Alpha-Ketoglutarate Dihydrate(2∶1) on intestinal immune function in severe intra bdominal infection rats

目的研究免疫营养素(鸟氨酸-α-酮戊二酸,OKG)强化的肠内营养对腹腔重症感染大鼠肠道免疫功能的影响。方法雄性SD大鼠20只,在行CLP术造成腹腔重症感染后随机分为普通肠内营养组(EN组,n=10)和肠内免疫营养组(EIN组,n=10)。EN组灌喂普通肠内营养液,EIN组灌喂等氮等热量的添加了鸟氨酸-α-酮戊二酸的肠内营养液(EIN)。2组营养剂糖、脂肪、蛋白质供热比为32%∶50%∶18%。术后早期开始灌喂并持续7 d,每日每只大鼠摄入能量标准量为882 k J·kg-1·d-1。7 d后麻醉处死两组大鼠,取全段小肠,计数总的肠集合淋巴结(PP结)细胞数,流式细胞仪检测PP结的T和B细胞的数目,光镜下观察肠黏膜组织形态学变化,测量肠绒毛高度。结果与普通肠内营养组相比,EIN组总的PP结细胞数、T细胞、B细胞数水平及空肠绒毛高度均高于EN组,差异均有统计学意义(P<0.05)。结论免疫营养物质OKG强化的肠内营养可以增加脓毒症模型大鼠的肠道PP结及T、B细胞数目,改善其肠道上皮组织结构,增强其免疫功能。

Objective To investigate the effects of immune nutrients （OKG） enriched enteral nutrition on intestinal immune function in severe intra bdominal infection rats. Methods After sepsis rats was induced by cecal ligation and puncture （CLP）, 20 male SD rats were randomly divided into normal enteral nutrition group （group EN, n=10） and enteral immune nutrition group （group EIN, n=10）. EN group was fed with normal enteral nutrition liquid. EIN group was given OKG-enriched enteral nutrition. The two groups rats received isocaloric, isonitrogenous, and isovolumic enteral nutrition. The relative proportions of energy in both groups were 32%carbohydrate, 50%fat and 18%protein. The energy requirement of each rat was calculated according to 882 kJ·kg^-1·d^-1. The rats were feed in the early postoperative period. Continuous feeding for 7 days, all rats were killed. Collected whole section intestine and counted total cell numbers in peyer’s patches. Used flow cytometry to measure the numbers of T and B cells in peyer’s patches. Changes in the morphology of intestinal mucosa was observed by electron microscopy. Measured the jejunum villus height. Results EIN group compared with the normal enteral nutrition group, the numbers of total cells, T and B cells in Peyer’s patches were significantly higher. The difference was statistical significance （P〈0.05）. Conclusion OKG-enriched enteral nutrition can increase the numbers of total cells, T and B cells in peyer’s patches in severe intra bdominal infection rats, improve the structure of intestinal epithelial tissue, improve the rats intestine immune function.