虎杖提取物对黄瓜防御酶系的影响

Effect of Extract of Polygonum cuspidatum on Defensive Enzymes of Cucumis sativus

本文采用热回流法提取虎杖乙醇提取物,测定不同浓度虎杖提取物处理下,黄瓜幼苗叶片中过氧化物酶（POD）、多酚氧化酶（PPO）、苯丙氨酸解氨酶（PAL）、几丁质酶（CHT）和β-1,3-葡聚糖酶（GLU）等活性的变化。结果表明,40 mg·L-1虎杖提取物处理下,黄瓜叶片POD和GLU（2~5 d）活性明显高于对照,PPO和CHT活性呈现M型变化趋势,且PPO、PAL和CHT活性与对照相比变化无规律。400 mg·L-1虎杖提取物处理的黄瓜幼苗叶片的POD、PPO和CHT活性明显高于40 mg·L-1的,但也一定程度上降低了GLU活性。

The ethanol extract was obtained from Polygonum cuspidatum by hot reflux extraction. The activities of peroxidase （POD）, polyphenol oxidase （PPO）, phenylalanine ammonialyase （PAL）, chitinase （CHT） and β-1,3-glucanase （GLU） in leaves of Cucumis sativus seedling were measured under different concentrations of extract ofP. cuspidatum. The results showed that under the treatment of 40 mg·L-1 extract, the activities of POD and GLU （2-5 d） of cucumber leaves were higher than those of control. And the activities of PPO and CHT showed a trend of M. Compared with the control, the activities of PPO, PAL and CHT were irregular. Under the treatment of 400 mg·L-1 extract, the activities of POD, PPO and CHT were obviously higher than those of 40 mg·L-1 extract, but the activity of GLU was decreased at a certain extent.