当归(粗)多糖对麻黄素肝组织损伤的保护作用

Protection of Angelica sinensis polysaccharide on liver histological structure in mice injured by ephedrine

目的探讨当归多糖对麻黄素致肝损伤的保护作用。方法 72只小鼠分为正常对照组、实验对照组、当归多糖1组和当归多糖2组4个组,实验对照组和当归多糖组连续腹腔注射0.2ml浓度为4.0g/L麻黄素溶液15d(每天2次),正常对照组注射等量生理盐水,当归多糖1组和2组在腹腔注射麻黄素溶液1h后,灌胃0.3ml(12.5g/L、25.0g/L)当归(粗)多糖溶液,正常对照组和实验对照组灌胃等量的生理盐水。分别于5、10、15d用光学显微镜技术观察各组小鼠肝组织结构的变化,用比色法检测小鼠血浆γ-谷氨酰转移酶(γ-GT)、碱性磷酸酶(AKP)、谷丙转氨酶(ALT)、谷草转氨酶(AST)及肝组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的变化,用免疫组织化学方法检测小鼠肝组织Bax蛋白、Csapase-3蛋白表达的变化。结果实验对照组小鼠肝组织有不同程度的损伤,肝细胞索排列紊乱,肝细胞疏松,肝血窦扩张,血浆γ-GT、AKP、ALT、AST活性明显升高,肝组织SOD活性显著降低,MDA含量显著升高,Bax、Caspase-3蛋白的表达显著升高;当归多糖组小鼠肝组织病理损伤明显减轻,血浆γ-GT、AKP、ALT、AST活性明显降低,肝组织SOD活性明显升高,MDA含量显著降低,Bax、Caspase-3蛋白的表达有所降低。结论当归(粗)多糖可提高肝组织细胞的抗氧化能力,抑制细胞凋亡,促进组织细胞损伤修复,对麻黄素致肝损伤具有一定的保护作用。

Objective To investigate the protection of Angelica sinensis polysaccharide （ASP） on ephedrine induced hepatic injury in mice. Methods Seventy-two mice were randomly divided into 4 groups： the natural control group,experiment control group, ASP1 group, and ASP2 group. The experimental control group and ASP groups were intraperitoneally injected with a 4. 0g/L of ephedrine 0.2ml twice a day for 15 days, and the natural control group was injected with a same amount of saline. The ASP groups were irrigated to stomach with 0.3ml Angelica sinensis polysaccharide solution （12.5g/L,25.0g/L）1 hour after ephedrine injection,the natural control group and experiment control group were irrigated to stomach with a same amount of saline. The mice were sacrificed at 5 day, 10day, and 15 day after treatment, respectively. The morphological observation was used to observe pathological changes of hepatic tissues. Activities of hepatic histologicalsuperoxide dismutase（ SOD）and plasma γ-glutamyhransferase（ γ-GT）, alkaline phosphatase （AKP）, glutamic- pyruvic transaminase （ALT） , glutamic-oxalacetic transaminase （AST） , as well as content of malonaldehyde （MDA） were detected by colorimetry. The expressions of Bax protein and Caspase-3 protein were measured by immunohistochemistry in liver of mice. Results There were various degree damages in hepatic tissues, hepatic cords were disorganized, liver cells were loose and liver sinusoid expanded, the activity of γ-GT, AKP, ALT, AST in plasma were significantly increased, the activity of SOD in liver tissue was significantly reduced,the content of MDA in liver tissue was increased significantly, the expression of Bax and Caspase-3 protein in liver were significantly increased. Compared with the experimental control group, pathological damage of liver was significantly improved, the activity of plasma γ-GT, AKP, ALT, AST were significantlyreduced,the activity of SOD in liver tissue was increased significantly, however the content of MDA was significantly decreased and the expressions of Bax and Caspase-3 protein in liver were reduced in Angelica sinensis polysaccharide group. Conclusion Angelica sinensis polysaccharide can improve liver tissue antioxidant capacity and promote reparation of cell injury and protect hepatic injury which is induced by ephedrine.