摘要
为水稻苗期耐冷性QTL定位克隆研究提供实验素材,以213个IR24/Asominori重组自交家系(RIL)为作图群体,并以141个SSR标记构建的分子连锁图谱为基础,结合苗期低温处理后亲本和213个RILs的苗期死苗率,运用QTL IciMapping 4.0软件进行水稻苗期耐冷性QTL检测及其遗传效应分析。结果表明:在第6、第11和第12染色体上检测到3个苗期耐冷性QTL,分别命名为qCTS-6、qCTS-11和qCTS-12。这3个QTL的LOD值分别为3.194 3、4.688 2和3.797,对应表型变异的解释率分别为5.662 7%、8.549 6%和12.787 7%,且抗性等位基因均来自苗期耐冷亲本Asominori。
To provided the experimental materials for identifying and cloning the quantitative trait loci （QTLs）of cold tolerance at the seedling stage,the authors analyzed QTLs location and evaluate the genetic effects of two parents and 213 RILs for cold tolerance at the seedling stage with the rate of dead seedlings by using software QTL IciMapping 4.0,and based on genetic linkage map of 141 SSR molecular markers and mapping population of 213 lines （recombination inbred lines,RIL）derived from a cross between IR24 and Asominori.The QTLs qCTS-6,qCTS-11andqCTS-12 related to cold tolerance at the seedling stage were detected on chromosome 6,11 and 12,respectively.Individual QTLs（LOD=3.194 3, LOD= 4.688 2,LOD = 3.797 0）explained 5.662 7%,8.549 6% and 12.787 7% of the observed phenotypic variance separately.All the three of detected QTLs allelescame from cold-tolerant parent Asominori.
出处
《贵州农业科学》
CAS
2015年第5期11-13,18,共4页
Guizhou Agricultural Sciences
基金
比尔和梅琳达.盖茨基金会项目"为非洲和亚洲资源贫瘠地区培育绿色超级稻-二期"(51587-15)
贵州省重大专项"水稻种质改良创新及新品种选育与应用"[黔科合重大专项字(2013)6023]
贵州省优秀科技教育人才省长专项基金项目"水稻耐冷性及品质性状基因(QTL)的分子定位与利用"[黔省专合字(2006)85]
贵州省科技创新人才团队项目"贵州省水稻遗传育种研究创新团队"[黔科合人才团队(2012)4020]
贵州省联合基金项目"水稻高密度遗传图谱构建和垩白QTL定位分析"[黔科合LH字(2014)7689]
贵州省农科院自主创新科研专项资金项目"水稻种质创新及高效育种与栽培技术体系研究"[农科院自主创新科研专项字(2014)019]
