壳聚糖浸种对盐胁迫苜蓿种子萌发受阻的缓解作用

Relieving Effects on Germination of Medicago sativa Seeds under Salt Stress Soaking in Chitosan

为解决苜蓿种子萌发的盐害问题,采用不同浓度壳聚糖溶液（50-250mmol/L）对苜蓿进行浸种处理,在100mmol/L NaCl胁迫下进行苜蓿种子的萌发试验。结果表明：与清水对照相比,盐胁迫显著抑制苜蓿种子的萌发,壳聚糖50-200mmol/L溶液浸种处理后,盐胁迫苜蓿种子的萌发效果均有不同程度升高,其中以150mg/L壳聚糖处理对盐胁迫下苜蓿种子萌发的缓解效果最好,其根长、活力指数、苗高、发芽指数、发芽势和发芽率分别比盐胁迫对照组高7.82倍、52.27%、47.92%、22.68%、16.28%和15.67%。

In order to solve problem of salt injury in germination of M.sativa seeds,the effects of seeds soaking in chitosan solutions with different concentrations （50 - 250 mmol/L ） on germination characteristics of the seeds of M.sativa under 100 mmol/L NaCl stress were studied.Results：The seed growth of M.sativa under salt stress were obviously inhibited compared with the control （water）.The seed germination indexes were improved in different extent after 50-200 mmol/L chitosan treatment,the treatment in 150 mmol/L chitosan could get the best relieving effect,the values of root length,vigor index,seedling length,germination index,germination energy,and germination percentage of the seeds in 150 mmol/L Chitosan improved 7.82 times,52.27%,47.92%,22.68%,16.28% and 15.67% higher than in the salt stress treatment respectively.