粗毛淫羊藿幼叶愈伤组织诱导及其总黄酮含量

Callus Induction of Epimedium acuminatum Young Leaves and Its Flavonoid Content

为了对粗毛淫羊藿野生资源的保护及淫羊藿黄酮离体生产体系提供技术参考,以粗毛淫羊藿幼叶为外植体,研究粗毛淫羊藿愈伤组织诱导的灭菌剂、消毒条件、培养基、激素配比和光照条件,并测定愈伤组织中总黄酮的含量。结果表明:利用粗毛淫羊藿幼叶进行愈伤组织培养,用2%的NaClO消毒14min,接种于培养基MS+2,4-D 2.0mg/L+6-BA 0.5mg/L+IBA 0.5mg/L+NAA 1mg/L,在24℃光照强度为1 000lx条件下进行诱导效果最好,继代和保存应在适宜的黑暗条件下进行;诱导出的胚性愈伤组织总黄酮含量较高,达5.38%,与野生粗毛淫羊藿总黄酮含量想当,高于药典规定的标准。

To provide technical references for protection of wild E.acuminatum resources and in vitro production system of flavonoids,the young leaves of E.acuminatum were used as explants for callus induction and its factors including different sterilization agent, medium, hormone combinations and illumination conditions which affect the callus induction of E.acuminatum were studied.Besides,the content of flavonoids in the calli was studied by means of the ultraviolet spectrophotometric analysis.The results indicate that the callus culture of E.acuminatum leaves can be induced under the following conditions：disinfection of 2% NaClO for 14 minutes,the medium MS ＋ 2,4-D 2.0 mg/L＋ 6-BA 0.5 mg/L＋ IBA 0.5 mg/L ＋ NAA 1 mg/L,at 24℃ 1 000 lx illumination conditions.The subculture and preservation is much appropriate under dark conditions.Total flavonoid content of induced embryonic calli is up to 5.38%,higher than that of pharmacopoeia standard.