生防放线菌的酶学特性及其代谢产物

Enzymatic Characteristics and Metabolites of Biocontrol Actinomycetes

为获得更多特异性各异的生防放线菌,采用皿内琼脂块法和试管斜面划线法,对从陕西省商洛市土壤分离的11株生防放线菌菌株(MX1,MX2,MX3,MX4,MX5,MX6,MX7,MX8,MX9,SY1,SY2)和供试细黄链霉菌进行生理生化试验。结果表明:有8株菌株产脂肪酶,SY1、MX1和细黄链霉菌的浑浊圈很明显,直径大于15mm的菌株共计4株;有10株菌株产生卵磷脂酶,直径大于15mm的菌株共计5株;有11株菌株对淀粉的水力较强,直径大于15mm的共计6株;仅4株菌株可水解酪蛋白。12株供试菌株革兰氏染色均呈阳性;V-P试验,MX1、MX2和SY1呈阳性,MX6呈弱阳性;甲基红试验,MX2、MX3和细黄链霉菌呈阳性,MX9呈弱阳性;共有9株菌株可使明胶液化,占供试菌株的75%;仅SY2产纤维素酶,有3株菌株产过氧化氢酶;有2株菌株能使牛奶培养基凝固,4株菌株能使其胨化。

The biophysical and biochemical test of 11 actinomycetes strains（MX1,MX2,MX3,MX4, MX5,MX6,MX7,MX8,MX9,SY1 and SY2）isolated from soil of Shangluo City in Shaanxi and tested Streptomyces microflavus was conducted by the agar block and test-tube slant streak plate methods to obtain more biocontrol actinomycetes with different specificities.Results：The diameter of hydrolyzation circle of 4 strains from 8 strains with producing lipase is more than 15 mm, and SY1, MX1 and Streptomyces microflavus strains have obvious hydrolyzation circles.The diameter of hydrolyzation circle of 5 strains from 10 strains with producing lecithinase is more than 15 mm.The diameter of hydrolyzation circle of 6 strains from 11 strains with strong starch hydrolysis capacity is more than 15 mm.4 strains can hydrolyze casein.12 tested strains present positive in gram stain test.MX1,MX2 and SY1 strains present positive and MX6 strain presents weakly positive in V-P test.MX2,MX3 and Streptomyces microflavus strains present positive and MX9 strain presents weakly positive in methyl red test.9 strains are of gelatin liquefaction capacity.Only SY2 strain can produce cellulose.3 strains can produce catalase.2 strains have the capacity of solidifying milk medium and 4 strains have the capacity of peptonizing milk.